ZFIN ID: ZDB-PUB-170207-2
Divergent effects of intrinsically active MEK variants on developmental Ras signaling
Goyal, Y., Jindal, G.A., Pelliccia, J.L., Yamaya, K., Yeung, E., Futran, A.S., Burdine, R.D., Schüpbach, T., Shvartsman, S.Y.
Date: 2017
Source: Nature Genetics   49(3): 465-469 (Journal)
Registered Authors: Burdine, Rebecca
Keywords: Diseases, Embryogenesis, Pattern formation
MeSH Terms:
  • Animals
  • Drosophila melanogaster/genetics
  • Germ-Line Mutation/genetics*
  • Heart Diseases/genetics
  • Humans
  • Mitogen-Activated Protein Kinases/genetics*
  • Neoplasms/genetics
  • Neurocognitive Disorders/genetics
  • Phenotype
  • Signal Transduction/genetics*
  • Zebrafish/genetics
  • ras Proteins/genetics*
PubMed: 28166211 Full text @ Nat. Genet.
ABSTRACT
Germline mutations in Ras pathway components are associated with a large class of human developmental abnormalities, known as RASopathies, that are characterized by a range of structural and functional phenotypes, including cardiac defects and neurocognitive delays. Although it is generally believed that RASopathies are caused by altered levels of pathway activation, the signaling changes in developing tissues remain largely unknown. We used assays with spatiotemporal resolution in Drosophila melanogaster (fruit fly) and Danio rerio (zebrafish) to quantify signaling changes caused by mutations in MAP2K1 (encoding MEK), a core component of the Ras pathway that is mutated in both RASopathies and cancers in humans. Surprisingly, we discovered that intrinsically active MEK variants can both increase and reduce the levels of pathway activation in vivo. The sign of the effect depends on cellular context, implying that some of the emerging phenotypes in RASopathies may be caused by increased, as well as attenuated, levels of Ras signaling.
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