PUBLICATION

Analysis of pancreatic disease in zebrafish

Authors
Eames Nalle, S.C., Franse, K.F., Kinkel, M.D.
ID
ZDB-PUB-170129-20
Date
2017
Source
Methods in cell biology   138: 271-295 (Chapter)
Registered Authors
Kinkel, Mary
Keywords
Blood glucose, Cell culture, Cold-water anesthesia, Diabetes, Dissection, Endocrine pancreas, Fasting, IP injection, Islet isolation, Weighing
MeSH Terms
  • Animals
  • Diabetes Mellitus/diagnostic imaging
  • Diabetes Mellitus/physiopathology
  • Disease Models, Animal
  • Gene Expression Regulation, Developmental
  • Humans
  • Insulin-Secreting Cells/pathology
  • Insulin-Secreting Cells/ultrastructure*
  • Molecular Imaging/methods*
  • Pancreas/physiopathology
  • Pancreas/ultrastructure*
  • Pancreatic Diseases/diagnostic imaging*
  • Pancreatic Diseases/physiopathology
  • Pancreatic Neoplasms/diagnostic imaging
  • Pancreatic Neoplasms/physiopathology
  • Zebrafish/genetics
PubMed
28129847 Full text @ Meth. Cell. Biol.
Abstract
One of the appeals of the zebrafish model is the relative ease of studying disease progression from embryonic or larval stages through to adulthood. Because of this, the zebrafish has become an important model for postembryonic pancreatic disease, particularly diabetes and pancreatic cancer. Here we present methods for using the adult zebrafish to analyze pancreas function and structure, with an emphasis on the endocrine pancreas and the beta cells. The methods include fasting, weighing adults, and anesthetizing adults, and intraperitoneal injection of glucose based on body weight. We also present dissection methods for removing the pancreas intact for histological studiesĀ and for sterile dissection of the principal islet followed by dissociation for cell culture-based studies of beta-cell function.
Genes / Markers
Figures
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping