PUBLICATION

One-Pot Microwave Synthesis of Water-Dispersible, High Fluorescence Silicon Nanoparticles and Their Imaging Applications in Vitro and in Vivo

Authors
Ye, H.L., Cai, S.J., Li, S., He, X.W., Li, W.Y., Li, Y.H., Zhang, Y.
ID
ZDB-PUB-161101-10
Date
2016
Source
Analytical chemistry   88(23): 11631-11638 (Journal)
Registered Authors
Li, Yuhao
Keywords
none
MeSH Terms
  • Animals
  • Fluorescence*
  • HeLa Cells
  • Humans
  • Microwaves*
  • Nanoparticles/chemistry*
  • Optical Imaging*
  • Silicon/chemistry*
  • Water/chemistry*
  • Zebrafish
PubMed
27797177 Full text @ Anal. Chem.
Abstract
Silicon nanoparticles (SiNPs) have been reported to be synthesized by microwave-assisted method under high pressure. However, there is still a lack of knowledge about the synthesis of SiNPs via microwave-assisted method under normal pressure. Here we developed a new facile one-pot microwave-assisted synthesis method of the excellent water-soluble SiNPs (~ 4.2 nm) under normal pressure by employing glycerol as the solvent. Meanwhile, glycerol might be responsible for the photoluminescence quantum yield (PLQY) value of the resultant SiNPs up to 47% for its nature of the organic solvent to contribute to yield less surface defects of the nanoparticles than those of being prepared in aqueous solution and thus to improve the fluorescent efficiency. The as-prepared SiNPs simultaneously featured bright blue-green fluorescence, long lifetime (~12.8 ns), obvious up-conversion luminescence originating from the two-photon absorption, superbly strong photo-stability and favorable low toxicity. As a satisfactory probe, the as-synthesized SiNPs were successfully applied in fluorescence imaging of human cervical carcinoma cell lines (HeLa) and zebrafish.
Genes / Markers
Figures
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping