PUBLICATION
Chemical Screening in Zebrafish
- Authors
- Brady, C.A., Rennekamp, A.J., Peterson, R.T.
- ID
- ZDB-PUB-160729-29
- Date
- 2016
- Source
- Methods in molecular biology (Clifton, N.J.) 1451: 3-16 (Chapter)
- Registered Authors
- Peterson, Randall
- Keywords
- Multi-well plates, Phenotypic screening, Small molecules
- MeSH Terms
-
- Animals
- Drug Discovery/methods
- Drug Evaluation, Preclinical/methods*
- Embryo, Nonmammalian/drug effects
- Embryo, Nonmammalian/physiology
- Larva/drug effects
- Larva/physiology
- Phenotype
- Small Molecule Libraries/pharmacology
- Zebrafish/embryology
- Zebrafish/physiology
- PubMed
- 27464797 Full text @ Meth. Mol. Biol.
Citation
Brady, C.A., Rennekamp, A.J., Peterson, R.T. (2016) Chemical Screening in Zebrafish. Methods in molecular biology (Clifton, N.J.). 1451:3-16.
Abstract
Phenotypic small molecule screens in zebrafish have gained popularity as an unbiased approach to probe biological processes. In this chapter we outline basic methods for performing chemical screens with larval zebrafish including breeding large numbers of embryos, plating larval fish into multi-well dishes, and adding small molecules to these wells. We also highlight important considerations when designing and interpreting the results of a phenotypic screen and possible follow-up approaches, including popular methods used to identify the mechanism of action of a chemical compound.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping