PUBLICATION
TALEN-Mediated Mutagenesis and Genome Editing
- Authors
- Ma, A.C., Chen, Y., Blackburn, P.R., Ekker, S.C.
- ID
- ZDB-PUB-160729-28
- Date
- 2016
- Source
- Methods in molecular biology (Clifton, N.J.) 1451: 17-30 (Chapter)
- Registered Authors
- Blackburn, Patrick, Chen, Yi, Ekker, Stephen C.
- Keywords
- Customized nucleases, FusX, Genome editing, Golden Gate, TALEN, Zebrafish
- MeSH Terms
-
- Animals
- CRISPR-Cas Systems/genetics
- DNA Breaks, Double-Stranded
- Gene Editing/methods*
- Mutagenesis/genetics*
- Transcription Activator-Like Effector Nucleases/genetics*
- Zebrafish/genetics
- PubMed
- 27464798 Full text @ Meth. Mol. Biol.
Citation
Ma, A.C., Chen, Y., Blackburn, P.R., Ekker, S.C. (2016) TALEN-Mediated Mutagenesis and Genome Editing. Methods in molecular biology (Clifton, N.J.). 1451:17-30.
Abstract
Transcription activator-like effectors (TALEs) are important genomic tools with customizable DNA-binding motifs for locus-specific modifications. In particular, TALE nucleases or TALENs have been successfully used in the zebrafish model system to introduce targeted mutations via repair of double-stranded breaks (DSBs) either through nonhomologous end joining (NHEJ) or by homology-directed repair (HDR) and homology-independent repair in the presence of a donor template. Compared with other customizable nucleases, TALENs offer high binding specificity and fewer sequence constraints in targeting the genome, with comparable mutagenic activity. Here, we describe a detailed in silico design tool for zebrafish genome editing for TALENs and CRISPR/Cas9 custom restriction enzymes using Mojo Hand 2.0 software.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping