PUBLICATION

Construction of the Inbred Strain

Authors
Shinya, M.
ID
ZDB-PUB-160729-22
Date
2016
Source
Methods in molecular biology (Clifton, N.J.)   1451: 107-18 (Chapter)
Registered Authors
Shinya, Minori
Keywords
Genetic homogeneity, Inbred strain, Inbreeding depression, Polymorphisms, Sib-pair mating, Zebrafish
MeSH Terms
  • Genetics, Population/methods*
  • Inbreeding
  • Polymorphism, Genetic/genetics*
PubMed
27464804 Full text @ Meth. Mol. Biol.
Abstract
Genetically homogeneous populations such as inbred strains are valuable experimental tools in various fields of biomedical analyses. In many animals, inbred strains are established by consecutive sib-pair mating for a minimum of 20 generations. As the generation proceeds, fitness of the population reduces usually. Therefore, in order to establish inbred strains, the important point is the selection of pairs in good condition at each generation. Here, I describe the procedure and tips for generating inbred strains in zebrafish.
Genes / Markers
Figures
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping