ZFIN ID: ZDB-PUB-160729-19
Imaging of Human Cancer Cell Proliferation, Invasion, and Micrometastasis in a Zebrafish Xenogeneic Engraftment Model
Tulotta, C., He, S., Chen, L., Groenewoud, A., van der Ent, W., Meijer, A.H., Spaink, H.P., Snaar-Jagalska, B.E.
Date: 2016
Source: Methods in molecular biology (Clifton, N.J.)   1451: 155-69 (Chapter)
Registered Authors: He, Shuning, Meijer, Annemarie H., Snaar-Jagalska, Ewa B., Spaink, Herman P., van der Ent, Wietske
Keywords: Chemical treatment, Metastasis, Microenvironment, Tumor invasion, Tumor proliferation, Xenotransplantation, Zebrafish embryo
MeSH Terms:
  • Animals
  • Cell Line, Tumor
  • Cell Proliferation/genetics
  • Cell Proliferation/physiology
  • Disease Models, Animal
  • Embryo, Nonmammalian/cytology
  • Embryo, Nonmammalian/metabolism
  • Embryo, Nonmammalian/pathology
  • Humans
  • Mice
  • Neoplasm Micrometastasis
  • Neoplasms/metabolism*
  • Neoplasms/pathology*
  • Transplantation, Heterologous
  • Zebrafish/embryology
  • Zebrafish/metabolism*
PubMed: 27464807 Full text @ Meth. Mol. Biol.
The xenograft model, using the early life stages of the zebrafish, allows imaging of tumor cell behavior both on a single cell and whole organism level, over time, within a week. This robust and reproducible assay can be used as an intermediate step between in vitro techniques and the expensive, and time consuming, murine models of cancer invasion and metastasis.In this chapter, a detailed protocol to inject human cancer cells into the blood circulation of a zebrafish embryo is described; the engraftment procedure is then followed by visualization and quantification methods of tumor cell proliferation, invasion, and micrometastasis formation during subsequent larval development. Interaction with the host microenvironment is also considered.