PUBLICATION

Imaging of Human Cancer Cell Proliferation, Invasion, and Micrometastasis in a Zebrafish Xenogeneic Engraftment Model

Authors
Tulotta, C., He, S., Chen, L., Groenewoud, A., van der Ent, W., Meijer, A.H., Spaink, H.P., Snaar-Jagalska, B.E.
ID
ZDB-PUB-160729-19
Date
2016
Source
Methods in molecular biology (Clifton, N.J.)   1451: 155-69 (Chapter)
Registered Authors
He, Shuning, Meijer, Annemarie H., Snaar-Jagalska, Ewa B., Spaink, Herman P., van der Ent, Wietske
Keywords
Chemical treatment, Metastasis, Microenvironment, Tumor invasion, Tumor proliferation, Xenotransplantation, Zebrafish embryo
MeSH Terms
  • Animals
  • Cell Line, Tumor
  • Cell Proliferation/genetics
  • Cell Proliferation/physiology
  • Disease Models, Animal
  • Embryo, Nonmammalian/cytology
  • Embryo, Nonmammalian/metabolism
  • Embryo, Nonmammalian/pathology
  • Humans
  • Mice
  • Neoplasm Micrometastasis
  • Neoplasms/metabolism*
  • Neoplasms/pathology*
  • Transplantation, Heterologous
  • Zebrafish/embryology
  • Zebrafish/metabolism*
PubMed
27464807 Full text @ Meth. Mol. Biol.
Abstract
The xenograft model, using the early life stages of the zebrafish, allows imaging of tumor cell behavior both on a single cell and whole organism level, over time, within a week. This robust and reproducible assay can be used as an intermediate step between in vitro techniques and the expensive, and time consuming, murine models of cancer invasion and metastasis.In this chapter, a detailed protocol to inject human cancer cells into the blood circulation of a zebrafish embryo is described; the engraftment procedure is then followed by visualization and quantification methods of tumor cell proliferation, invasion, and micrometastasis formation during subsequent larval development. Interaction with the host microenvironment is also considered.
Genes / Markers
Figures
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping