PUBLICATION
Imaging of Human Cancer Cell Proliferation, Invasion, and Micrometastasis in a Zebrafish Xenogeneic Engraftment Model
- Authors
- Tulotta, C., He, S., Chen, L., Groenewoud, A., van der Ent, W., Meijer, A.H., Spaink, H.P., Snaar-Jagalska, B.E.
- ID
- ZDB-PUB-160729-19
- Date
- 2016
- Source
- Methods in molecular biology (Clifton, N.J.) 1451: 155-69 (Chapter)
- Registered Authors
- He, Shuning, Meijer, Annemarie H., Snaar-Jagalska, Ewa B., Spaink, Herman P., van der Ent, Wietske
- Keywords
- Chemical treatment, Metastasis, Microenvironment, Tumor invasion, Tumor proliferation, Xenotransplantation, Zebrafish embryo
- MeSH Terms
-
- Animals
- Cell Line, Tumor
- Cell Proliferation/genetics
- Cell Proliferation/physiology
- Disease Models, Animal
- Embryo, Nonmammalian/cytology
- Embryo, Nonmammalian/metabolism
- Embryo, Nonmammalian/pathology
- Humans
- Mice
- Neoplasm Micrometastasis
- Neoplasms/metabolism*
- Neoplasms/pathology*
- Transplantation, Heterologous
- Zebrafish/embryology
- Zebrafish/metabolism*
- PubMed
- 27464807 Full text @ Meth. Mol. Biol.
Citation
Tulotta, C., He, S., Chen, L., Groenewoud, A., van der Ent, W., Meijer, A.H., Spaink, H.P., Snaar-Jagalska, B.E. (2016) Imaging of Human Cancer Cell Proliferation, Invasion, and Micrometastasis in a Zebrafish Xenogeneic Engraftment Model. Methods in molecular biology (Clifton, N.J.). 1451:155-69.
Abstract
The xenograft model, using the early life stages of the zebrafish, allows imaging of tumor cell behavior both on a single cell and whole organism level, over time, within a week. This robust and reproducible assay can be used as an intermediate step between in vitro techniques and the expensive, and time consuming, murine models of cancer invasion and metastasis.In this chapter, a detailed protocol to inject human cancer cells into the blood circulation of a zebrafish embryo is described; the engraftment procedure is then followed by visualization and quantification methods of tumor cell proliferation, invasion, and micrometastasis formation during subsequent larval development. Interaction with the host microenvironment is also considered.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping