PUBLICATION

Live Imaging of Host-Pathogen Interactions in Zebrafish Larvae

Authors
Matty, M.A., Oehlers, S.H., Tobin, D.M.
ID
ZDB-PUB-160729-16
Date
2016
Source
Methods in molecular biology (Clifton, N.J.)   1451: 207-23 (Chapter)
Registered Authors
Matty, Molly, Oehlers, Stefan, Tobin, David
Keywords
Drug treatment, Infection, Innate immunity, Mycobacteria, Time-lapse imaging, Zebrafish
MeSH Terms
  • Animals
  • Host-Pathogen Interactions/genetics
  • Host-Pathogen Interactions/immunology
  • Host-Pathogen Interactions/physiology*
  • Immunity, Innate/genetics
  • Immunity, Innate/physiology
  • Larva/immunology*
  • Larva/metabolism*
  • Larva/microbiology
  • Mycobacterium marinum/immunology
  • Mycobacterium marinum/pathogenicity
  • Zebrafish/immunology*
  • Zebrafish/metabolism*
  • Zebrafish/microbiology
PubMed
27464810 Full text @ Meth. Mol. Biol.
Abstract
Zebrafish larvae are a powerful platform for studying the innate immune response to infection. The small size and optical transparency of larval zebrafish allow for multiple subject, multidimensional, and longitudinal imaging experiments. This chapter describes protocols for infecting zebrafish larvae with their natural pathogen Mycobacterium marinum, rapid short-term imaging, long-term extended imaging, and drug treatment assays. These protocols can be easily adapted to image and manipulate host interactions with other pathogens.
Genes / Markers
Figures
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping