PUBLICATION

Heavy chain (LvH) and light chain (LvL) of lipovitellin (Lv) of zebrafish can both bind to bacteria and enhance phagocytosis

Authors
Liang, X., Hu, Y., Feng, S., Zhang, S., Zhang, Y., Sun, C.
ID
ZDB-PUB-160519-25
Date
2016
Source
Developmental and comparative immunology   63: 47-55 (Journal)
Registered Authors
Zhang, Yu
Keywords
Danio rerio, Lipovitellin, Maternal immunity, Zebrafish
MeSH Terms
  • Animals
  • Bacterial Infections/immunology*
  • Cells, Cultured
  • Egg Proteins/genetics
  • Egg Proteins/metabolism*
  • Escherichia coli/immunology
  • Fish Proteins/genetics
  • Fish Proteins/metabolism*
  • Immunity, Maternally-Acquired
  • Lipopolysaccharides/metabolism
  • Macrophages/immunology*
  • Macrophages/microbiology
  • Micrococcus luteus/immunology
  • Pathogen-Associated Molecular Pattern Molecules/immunology
  • Phagocytosis
  • Protein Subunits/genetics
  • Protein Subunits/metabolism*
  • Staphylococcus aureus/immunology
  • Zebrafish/immunology*
PubMed
27185202 Full text @ Dev. Comp. Immunol.
Abstract
Lipovitellin (Lv) is an apoprotein in oviparous animals. Lv consists of a heavy chain (LvH) and a light chain (LvL) which are traditionally regarded as energy reserves for developing embryos. Recently, Lv has been shown to be involved in immune defense of developing embryos in fish. However, it remains unknown if each of LvH and LvL possesses immune activity; and if so, whether or not they function similarly. Here we clearly demonstrated that recombinant LvH (rLvH) and LvL (rLvL) from zebrafish vg1 gene bound to both the Gram-negative bacteria Escherichia coli and Vibrio anguillarum and the Gram-positive bacteria Staphylococcus aureus and Micrococcus luteus as well as the pathogen-associated molecular patterns LPS, LTA and PGN. In addition, both rLvH and rLvL were able to enhance the phagocytosis of bacteria E. coli and S. aureus by macrophages. All these data suggest that both LvH and LvL, in addition to being energy reserves, are also maternal immune-relevant factors capable of interacting with invading bacteria in zebrafish embryos/larvae.
Genes / Markers
Figures
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping