PUBLICATION

Hormetic effect induced by depleted uranium in zebrafish embryos

Authors
Ng, C.Y., Cheng, S.H., Yu, K.N.
ID
ZDB-PUB-160411-3
Date
2016
Source
Aquatic toxicology (Amsterdam, Netherlands)   175: 184-191 (Journal)
Registered Authors
Cheng, Shuk Han
Keywords
Depleted uranium, Hormetic effect, Uranium, Zebrafish embryo
MeSH Terms
  • Animals
  • Apoptosis/drug effects*
  • Embryo, Nonmammalian/drug effects
  • Hormesis/drug effects*
  • Uranium/toxicity*
  • Water Pollutants, Chemical/toxicity
  • Zebrafish/physiology*
PubMed
27060238 Full text @ Aquat. Toxicol.
Abstract
The present work studied the hormetic effect induced by uranium (U) in embryos of zebrafish (Danio rerio) using apoptosis as the biological endpoint. Hormetic effect is characterized by biphasic dose-response relationships showing a low-dose stimulation and a high-dose inhibition. Embryos were dechorionated at 4h post fertilization (hpf), and were then exposed to 10 or 100μg/l depleted uranium (DU) in uranyl acetate solutions from 5 to 6 hpf. For exposures to 10μg/l DU, the amounts of apoptotic signals in the embryos were significantly increased at 20 hpf but were significantly decreased at 24 hpf, which demonstrated the presence of U-induced hormesis. For exposures to 100μg/l DU, the amounts of apoptotic signals in the embryos were significantly increased at 20, 24 and 30 hpf. Hormetic effect was not shown but its occurrence between 30 and 48 hpf could not be ruled out. In conclusion, hormetic effect could be induced in zebrafish embryos in a concentration- and time-dependent manner.
Genes / Markers
Figures
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping