PUBLICATION

Imaging of living cells and zebrafish in vivo using a ratiometric fluorescent probe for hydrogen sulfide

Authors
Liu, T., Lin, J., Li, Z., Lin, L., Shen, Y., Zhu, H., Qian, Y.
ID
ZDB-PUB-150925-2
Date
2015
Source
The Analyst   140(21): 7165-9 (Journal)
Registered Authors
Li, Zhen
Keywords
none
MeSH Terms
  • Animals
  • Cyclohexanones/chemistry
  • Fluorescent Dyes/chemistry*
  • HeLa Cells
  • Humans
  • Hydrogen Sulfide/chemistry*
  • Microscopy, Confocal
  • Microscopy, Fluorescence*
  • Models, Chemical
  • Nitriles/chemistry*
  • Zebrafish
PubMed
26401525 Full text @ Analyst
Abstract
We have developed a novel colorimetric and ratiometric fluorescence probe for the selective and sensitive monitoring of hydrogen sulfide based on a dicyanoisophorone platform. An excellent linear relationship of fluorescence intensity ratio (I637/I558) (R(2) = 0.9867) versus hydrogen sulfide concentration in the range of 1-12 μM was obtained. This probe exhibited a remarkable fluorescence response to hydrogen sulfide over other physiological thiols or biological species, which fluoresces in the red region with a large Stokes shift (172 nm). This probe was successfully utilized to monitor H2S under in vitro physiological conditions and for imaging H2S in living cells and living zebrafish in vivo.
Genes / Markers
Figures
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping