PUBLICATION

Kidney Regeneration in Adult Zebrafish by Gentamicin Induced Injury

Authors
Kamei, C.N., Liu, Y., Drummond, I.A.
ID
ZDB-PUB-150815-2
Date
2015
Source
Journal of visualized experiments : JoVE   (102): e51912 (Journal)
Registered Authors
Drummond, Iain, Liu, Yan
Keywords
Developmental biology, kidney, mesonephros, gentamicin, injection, zebrafish, injury, regeneration, Lhx1a, heat shock, adult
MeSH Terms
  • Acute Kidney Injury/chemically induced*
  • Animals
  • Female
  • Gentamicins/administration & dosage*
  • Injections, Intraperitoneal
  • Kidney/drug effects*
  • Kidney/physiology*
  • Male
  • Nephrons/drug effects
  • Nephrons/physiology
  • Regeneration/drug effects*
  • Regeneration/physiology*
  • Stem Cells/drug effects
  • Stem Cells/physiology
  • Zebrafish/physiology*
PubMed
26275011 Full text @ J. Vis. Exp.
Abstract
The kidney is essential for fluid homeostasis, blood pressure regulation and filtration of waste from the body. The fundamental unit of kidney function is the nephron. Mammals are able to repair existing nephrons after injury, but lose the ability to form new nephrons soon after birth. In contrast to mammals, adult fish produce new nephrons (neonephrogenesis) throughout their lives in response to growth requirements or injury. Recently, lhx1a has been shown to mark nephron progenitor cells in the adult zebrafish kidney, however mechanisms controlling the formation of new nephrons after injury remain unknown. Here we show our method for robust and reproducible injury in the adult zebrafish kidney by intraperitoneal (i.p.) injection of gentamicin, which uses a noninvasive visual screening process to select for fish with strong but nonlethal injury. Using this method, we can determine optimal gentamicin dosages for injury and go on to demonstrate the effect of higher temperatures on kidney regeneration in zebrafish.
Genes / Markers
Figures
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping