PUBLICATION

Individual Cell Migration Serves as the Driving Force for Optic Vesicle Evagination

Authors
Rembold, M., Loosli, F., Adams, R. J., Wittbrodt, J.
ID
ZDB-PUB-150422-16
Date
2006
Source
Science (New York, N.Y.)   313: 1130-1134 (Journal)
Registered Authors
Adams, Richard, Loosli, Felix, Rembold, Martina, Wittbrodt, Jochen
Keywords
none
MeSH Terms
  • Animals
  • Animals, Genetically Modified
  • Cell Movement*
  • Cell Shape
  • Central Nervous System/embryology
  • Epithelial Cells/cytology
  • Epithelial Cells/physiology
  • Eye/embryology*
  • Fish Proteins/genetics
  • Fish Proteins/physiology
  • Gastrula/cytology
  • Homeodomain Proteins/genetics
  • Homeodomain Proteins/physiology
  • Image Processing, Computer-Assisted
  • Microscopy, Confocal
  • Morphogenesis
  • Mutation
  • Oryzias/embryology*
  • Oryzias/genetics
  • Prosencephalon/embryology
  • Retina/cytology
  • Retina/embryology*
  • Stem Cell Transplantation
  • Stem Cells/physiology*
PubMed
16931763 Full text @ Science
Abstract
The cellular mechanisms underlying organ formation are largely unknown. We visualized early vertebrate eye morphogenesis at single-cell resolution by in vivo imaging in medaka (Oryzias latipes). Before optic vesicle evagination, retinal progenitor cells (RPCs) modulate their convergence in a fate-specific manner. Presumptive forebrain cells converge toward the midline, whereas medial RPCs remain stationary, predetermining the site of evagination. Subsequent optic vesicle evagination is driven by the active migration of individual RPCs. The analysis of mutants demonstrated that the retina-specific transcription factor rx3 determines the convergence and migration behaviors of RPCs. Hence, the migration of individual cells mediates essential steps of organ morphogenesis.
Genes / Markers
Figures
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping