PUBLICATION

Notch signaling regulates venous arterialization during zebrafish fin regeneration

Authors
Kametani, Y., Chi, N.C., Stainier, D.Y., Takada, S.
ID
ZDB-PUB-150327-5
Date
2015
Source
Genes to cells : devoted to molecular & cellular mechanisms   20(5): 427-38 (Journal)
Registered Authors
Chi, Neil C., Kametani, Yoshiko, Stainier, Didier, Takada, Shinji
Keywords
none
MeSH Terms
  • Animal Fins/physiology*
  • Animals
  • Neovascularization, Physiologic*
  • Receptors, Notch/metabolism*
  • Regeneration*
  • Signal Transduction*
  • Vascular Remodeling
  • Zebrafish/physiology*
PubMed
25810153 Full text @ Genes Cells
Abstract
To protect against blood pressure, a mature artery is supported by mural cells which include vascular smooth muscle cells and pericytes. To regenerate a functional vascular system, arteries should be properly reconstructed with mural cells although the mechanisms underlying artery reconstruction remain unclear. In this study, we examined the process of artery reconstruction during regeneration of the zebrafish caudal fin as a model to study arterial formation in an adult setting. During fin regeneration, the arteries and veins form a net-like vasculature called the vascular plexus, and this plexus undergoes remodeling to form a new artery and two flanking veins. We found that the new vascular plexus originates mainly from venous cells in the stump but very rarely from the arterial cells. Interestingly, these vein-derived cells contributed to the reconstructed arteries. This arterialization was dependent on Notch signaling, and further analysis showed that Notch signaling was required for the initiation of arterial gene expression. In contrast, venous remodeling did not require Notch signaling. These results provide new insights toward understanding mechanisms of vascular regeneration and illustrate the utility of the adult zebrafish fin to study this process.
Genes / Markers
Figures
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping