PUBLICATION

Capturing Tissue Repair in Zebrafish Larvae with Time-lapse Brightfield Stereomicroscopy

Authors
Lisse, T.S., Brochu, E.A., Rieger, S.
ID
ZDB-PUB-150306-5
Date
2015
Source
Journal of visualized experiments : JoVE   (95): (Journal)
Registered Authors
Rieger, Sandra
Keywords
none
MeSH Terms
  • Animals
  • Microscopy/methods
  • Regeneration/physiology*
  • Tail/physiology
  • Zebrafish/physiology*
PubMed
25742070 Full text @ J. Vis. Exp.
Abstract
The zebrafish larval tail fin is ideal for studying tissue regeneration due to the simple architecture of the larval fin-fold, which comprises of two layers of skin that enclose undifferentiated mesenchyme, and because the larval tail fin regenerates rapidly within 2-3 days. Using this system, we demonstrate a method for capturing the repair dynamics of the amputated tail fin with time-lapse video brightfield stereomicroscopy. We demonstrate that fin amputation triggers a contraction of the amputation wound and extrusion of cells around the wound margin, leading to their subsequent clearance. Fin regeneration proceeds from proximal to distal direction after a short delay. In addition, developmental growth of the larva can be observed during all stages. The presented method provides an opportunity for observing and analyzing whole tissue-scale behaviors such as fin development and growth in a simple microscope setting, which is easily adaptable to any stereomicroscope with time-lapse capabilities.
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