PUBLICATION

Imaging the Zebrafish Dentition: From Traditional Approaches to Emerging Technologies

Authors
Bruneel, B., Mathä, M., Paesen, R., Ameloot, M., Weninger, W.J., Huysseune, A.
ID
ZDB-PUB-150107-4
Date
2015
Source
Zebrafish   12(1): 1-10 (Review)
Registered Authors
Huysseune, Ann
Keywords
none
MeSH Terms
  • Animals
  • Dentition*
  • Microscopy
  • Microtomy
  • Staining and Labeling
  • Tomography
  • Tooth/anatomy & histology
  • Tooth/growth & development
  • Zebrafish/anatomy & histology*
  • Zebrafish/growth & development
PubMed
25560992 Full text @ Zebrafish
Abstract
Abstract The zebrafish, a model organism for which a plethora of molecular and genetic techniques exists, has a lifelong replacing dentition of 22 pharyngeal teeth. This is in contrast to the mouse, which is the key organism in dental research but whose teeth are never replaced. Employing the zebrafish as the main organism to elucidate the mechanisms of continuous tooth replacement, however, poses at least one major problem, related to the fact that all teeth are located deep inside the body. Investigating tooth replacement thus relies on conventional histological methods, which are often laborious, time-consuming and can cause tissue deformations. In this review, we investigate the advantages and limitations of adapting current visualization techniques to dental research in zebrafish. We discuss techniques for fast sectioning, such as vibratome sectioning and high-resolution episcopic microscopy, and methods for in toto visualization, such as Alizarin red staining, micro-computed tomography, and optical projection tomography. Techniques for in vivo imaging, such as two-photon excitation fluorescence and second harmonic generation microscopy, are also covered. Finally, the possibilities of light sheet microscopy are addressed.
Genes / Markers
Figures
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping