PUBLICATION
Serotonin antagonists induce anxiolytic and anxiogenic-like behaviour in zebrafish in a receptor-subtype dependent manner
- Authors
- Nowicki, M., Tran, S., Muraleetharan, A., Markovic, S., Gerlai, R.
- ID
- ZDB-PUB-141007-6
- Date
- 2014
- Source
- Pharmacology, biochemistry, and behavior 126: 170-80 (Journal)
- Registered Authors
- Gerlai, Robert T.
- Keywords
- Anxiety, Behaviour, Novel environment, Serotonin antagonist, Zebrafish
- MeSH Terms
-
- Aminopyridines/pharmacology
- Animals
- Anti-Anxiety Agents/pharmacology*
- Anti-Anxiety Agents/therapeutic use
- Anxiety/chemically induced*
- Anxiety/drug therapy
- Benzamides/pharmacology
- Ketanserin/pharmacology
- Ondansetron/pharmacology
- Piperazines/pharmacology
- Pyridines/pharmacology
- Receptors, Serotonin/metabolism*
- Serotonin 5-HT1 Receptor Antagonists/pharmacology*
- Serotonin 5-HT2 Receptor Antagonists/pharmacology*
- Serotonin 5-HT3 Receptor Antagonists/pharmacology*
- Zebrafish
- PubMed
- 25284132 Full text @ Pharmacol. Biochem. Behav.
Citation
Nowicki, M., Tran, S., Muraleetharan, A., Markovic, S., Gerlai, R. (2014) Serotonin antagonists induce anxiolytic and anxiogenic-like behaviour in zebrafish in a receptor-subtype dependent manner. Pharmacology, biochemistry, and behavior. 126:170-80.
Abstract
Motor function and anxiety-like responses are easily quantifiable in zebrafish, a novel model organism for behavioural pharmacology. Activation of serotonin receptors through the use of selective agonists have been shown to alter anxiety-like behaviours in zebrafish. However, few studies have examined the effect of blockade of specific serotonin receptors. In the current study, we examine the effect of 4 serotonin receptor antagonists selective for 5-HT1A, 5-HT1B/D, 5-HT2, and 5-HT3 receptors on zebrafish motor and anxiety-like responses. Exposure to the receptor antagonists did not change baseline motor responses. However, when placed in a novel environment, zebrafish previously exposed to GR 55562 (5-HT1B/D antagonist) exhibited reduced anxiety-like behaviour, whereas zebrafish previously exposed to p-MPPF (5-HT1A antagonist), Ketanserin (5-HT2 antagonist), or Ondasetron (5-HT3 antagonist) exhibited increased anxiety-like behaviours. These results show that drugs developed for mammalian serotonin receptors are efficacious in the zebrafish too, a finding that demonstrates evolutionary conservation of the serotoninergic system. The results also imply that zebrafish may be an appropriate animal model for examining the serotonergic neurotransmitter system in vertebrates.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping