PUBLICATION
Differential effects of dopamine D1 and D 2/3 receptor antagonism on motor responses
- Authors
- Tran, S., Nowicki, M., Muraleetharan, A., Gerlai, R.
- ID
- ZDB-PUB-140820-11
- Date
- 2015
- Source
- Psychopharmacology 232(4): 795-806 (Journal)
- Registered Authors
- Gerlai, Robert T.
- Keywords
- none
- MeSH Terms
-
- Animals
- Benzazepines/pharmacology*
- Dopamine/metabolism
- Dopamine Antagonists/pharmacology*
- Dopamine D2 Receptor Antagonists/pharmacology*
- Dose-Response Relationship, Drug
- Male
- Motor Activity/drug effects*
- Motor Activity/physiology
- Random Allocation
- Receptors, Dopamine D1/antagonists & inhibitors*
- Receptors, Dopamine D1/metabolism
- Receptors, Dopamine D2/metabolism
- Receptors, Dopamine D3/antagonists & inhibitors*
- Receptors, Dopamine D3/metabolism
- Sulpiride/analogs & derivatives*
- Sulpiride/pharmacology
- Zebrafish
- PubMed
- 25134500 Full text @ Psychpharma
Citation
Tran, S., Nowicki, M., Muraleetharan, A., Gerlai, R. (2015) Differential effects of dopamine D1 and D 2/3 receptor antagonism on motor responses. Psychopharmacology. 232(4):795-806.
Abstract
Rationale The zebrafish dopaminergic system is thought to be evolutionarily conserved and may be amenable to pharmacological manipulation using drugs developed for mammalian receptors. However, only few studies have examined the role of specific receptor subtypes in behaviour of adult zebrafish.
Objectives The objectives of this study are to determine the translational relevance of the zebrafish and examine the psychopharmacology of specific dopamine receptors in this species.
Methods Using a behavioural pharmacological approach, we examine the effect of D1 and D2/3 receptor antagonisms on motor patterns of adult zebrafish during acute drug exposure and withdrawal.
Results Acute exposure to SCH-23390 (D1 receptor antagonist) decreased total distance travelled in a dose-dependent manner. Exposure to amisulpride (D2/3 receptor antagonist) induced a biphasic dose-response in total distance travelled and in angular velocity. The results provide support for the existence of structurally and functionally conserved postsynaptic D1 and D2 receptors, as well as presynaptic D2 autoreceptors in the zebrafish brain. The behavioural effects of the employed antagonists did not persist following 30 min of withdrawal.
Conclusion The results suggest that zebrafish, a cheaper and simpler model organism compared to the rat and the mouse, may be an efficient translationally relevant tool for the analysis of the psychopharmacology of receptors of the vertebrate dopaminergic system.
Genes / Markers
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Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping