PUBLICATION
OECD validation study to assess intra- and inter-laboratory reproducibility of the zebrafish embryo toxicity test for acute aquatic toxicity testing
- Authors
- Busquet, F., Strecker, R., Rawlings, J.M., Belanger, S.E., Braunbeck, T., Carr, G.J., Cenijn, P., Fochtman, P., Gourmelon, A., Hübler, N., Kleensang, A., Knöbel, M., Kussatz, C., Legler, J., Lillicrap, A., Martínez-Jerónimo, F., Polleichtner, C., Rzodeczko, H., Salinas, E., Schneider, K.E., Scholz, S., Brandhof, E.J., van der Ven, L.T., Walter-Rohde, S., Weigt, S., Witters, H., Halder, M.
- ID
- ZDB-PUB-140531-12
- Date
- 2014
- Source
- Regulatory toxicology and pharmacology : RTP 69(3): 496-511 (Journal)
- Registered Authors
- Braunbeck, Thomas, Legler, Juliette, van der Ven, Leo, Witters, Hilda
- Keywords
- Alternative method, Aquatic acute toxicity, Reproducibility, Validation, Zebrafish embryo
- MeSH Terms
-
- Animals
- Laboratories
- Lethal Dose 50
- Organisation for Economic Co-Operation and Development
- Reproducibility of Results
- Toxicity Tests, Acute/methods*
- Water Pollutants, Chemical/toxicity*
- Zebrafish
- PubMed
- 24874798 Full text @ Regul. Toxicol. Pharmacol.
Citation
Busquet, F., Strecker, R., Rawlings, J.M., Belanger, S.E., Braunbeck, T., Carr, G.J., Cenijn, P., Fochtman, P., Gourmelon, A., Hübler, N., Kleensang, A., Knöbel, M., Kussatz, C., Legler, J., Lillicrap, A., Martínez-Jerónimo, F., Polleichtner, C., Rzodeczko, H., Salinas, E., Schneider, K.E., Scholz, S., Brandhof, E.J., van der Ven, L.T., Walter-Rohde, S., Weigt, S., Witters, H., Halder, M. (2014) OECD validation study to assess intra- and inter-laboratory reproducibility of the zebrafish embryo toxicity test for acute aquatic toxicity testing. Regulatory toxicology and pharmacology : RTP. 69(3):496-511.
Abstract
The OECD validation study of the zebrafish embryo acute toxicity test (ZFET) for acute aquatic toxicity testing evaluated the ZFET reproducibility by testing 20 chemicals at 5 different concentrations in 3 independent runs in at least 3 laboratories. Stock solutions and test concentrations were analytically confirmed for 11 chemicals. Newly fertilised zebrafish eggs (20/concentration and control) were exposed for 96h to chemicals. Four apical endpoints were recorded daily as indicators of acute lethality: coagulation of the embryo, lack of somite formation, non-detachment of the tail bud from the yolk sac and lack of heart-beat. Results (LC50 values for 48/96h exposure) show that the ZFET is a robust method with a good intra- and inter-laboratory reproducibility (CV < 30%) for most chemicals and laboratories. The reproducibility was lower (CV > 30%) for some very toxic or volatile chemicals, and chemicals tested close to their limit of solubility. The ZFET is now available as OECD Test Guideline 236. Considering the high predictive capacity of the ZFET demonstrated by Belanger et al (2013, ETC 32:1768-1783) in their retrospective analysis of acute fish toxicity and fish embryo acute toxicity data, the ZFET is ready to be considered for acute fish toxicity for regulatory purposes.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping