PUBLICATION

TALEN construction via "Unit Assembly" method and targeted genome modifications in zebrafish

Authors
Huang, P., Xiao, A., Tong, X., Zu, Y., Wang, Z., Zhang, B.
ID
ZDB-PUB-140513-497
Date
2014
Source
Methods (San Diego, Calif.)   69(1): 67-75 (Journal)
Registered Authors
Tong, Xiangjun, Wang, Zhanxiang, Xiao, An, Zhang, Bo, Zu, Yao
Keywords
Chromosomal deletion, Gene targeting, Genome manipulation, Homologous recombination, TALEN, Zebrafish
MeSH Terms
  • Animals
  • Chromosome Deletion
  • Deoxyribonucleases/chemistry*
  • Deoxyribonucleases/genetics
  • Gene Knock-In Techniques
  • Gene Knockout Techniques
  • Genetic Engineering/methods*
  • Germ-Line Mutation
  • Homologous Recombination
  • Protein Engineering/methods
  • Zebrafish/genetics
PubMed
24556555 Full text @ Methods
Abstract
Transcription activator-like effector nucleases (TALENs) are engineered endonucleases composed of a customized transcription activator-like effector (TALE) DNA-binding domain and a FokI DNA cleavage domain. TALENs induce DNA double-strand breaks (DSBs) at their target sites on the chromosome and have been successfully used for genome engineering in many species and cultured cells. Zebrafish is a very popular model organism in both basic and clinical research. Here, we describe the details of construction of customized TALENs using the "Unit Assembly" (UA) method, as well as three applications of zebrafish genome manipulations using TALENs: gene knock-out, large chromosome deletion, and gene knock-in by homologous recombination.
Genes / Markers
Figures
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping