PUBLICATION

Long-term disruption of growth, reproduction, and behavior after embryonic exposure of zebrafish to PAH-spiked sediment

Authors
Vignet, C., Devier, M.H., Le Menach, K., Lyphout, L., Potier, J., Cachot, J., Budzinski, H., Bégout, M.L., Cousin, X.
ID
ZDB-PUB-140513-289
Date
2014
Source
Environmental science and pollution research international   21(24): 13877-87 (Journal)
Registered Authors
Cousin, Xavier
Keywords
none
MeSH Terms
  • Animals
  • Behavior, Animal/drug effects*
  • Embryo, Nonmammalian/drug effects
  • Female
  • Geologic Sediments/chemistry*
  • Male
  • Polycyclic Aromatic Hydrocarbons/analysis
  • Polycyclic Aromatic Hydrocarbons/toxicity*
  • Reproduction/drug effects
  • Water Pollutants, Chemical/analysis
  • Water Pollutants, Chemical/toxicity*
  • Zebrafish/growth & development*
  • Zebrafish/physiology
PubMed
24659400 Full text @ Environ. Sci. Pollut. Res. Int.
Abstract
A natural sediment spiked with three individual polycyclic aromatic hydrocarbons (PAHs; pyrene, phenanthrene and benzo[a]pyrene) was used to expose zebrafish embryos and larvae during 4 days. The total PAH concentration was 4.4 μg g(-1) which is in the range found in sediment from contaminated areas. Quantification of metabolites in the larvae after exposure confirmed the actual contamination of the larvae and indicated an active metabolism especially for pyrene and benzo[a]pyrene. After a transfer in a clean medium, the larvae were reared to adulthood and evaluated for survival, growth, reproduction, and behavior. Measured endpoints revealed a late disruption of growth (appearing at 5 months) and a trend toward a lower reproductive ability. Adults of embryos exposed to sediment spiked with PAHs displayed lethargic and/or anxiety-like behaviors. This latter behavior was also identified in offspring at larval stage. All together, these effects could have detrimental consequences on fish performances and contribution to recruitment.
Genes / Markers
Figures
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping