PUBLICATION

Dynamin-dependent maintenance of epithelial integrity is essential for zebrafish epiboly

Authors
Lepage, S.E., and Bruce, A.E.
ID
ZDB-PUB-140502-9
Date
2014
Source
Bioarchitecture   4(1): 31-34 (Other)
Registered Authors
Bruce, Ashley, Lepage, Stephanie
Keywords
ERM proteins, dynamin, endocytosis, enveloping layer, epiboly, epithelial integrity, synaptotagmin, zebrafish
MeSH Terms
  • Actomyosin/metabolism*
  • Animals
  • Blastoderm/embryology*
  • Dynamins/metabolism*
  • Zebrafish/embryology*
PubMed
24522009 Full text @ Bioarchitecture
Abstract

Epiboly, the thinning and spreading of one tissue over another, is a widely employed morphogenetic movement that is essential for the development of many organisms. In the zebrafish embryo, epiboly describes the coordinated vegetal movement of the deep cells, enveloping layer (EVL) and yolk syncytial layer (YSL) to engulf the yolk cell. Recently, we showed that the large GTPase Dynamin plays a fundamental role in epiboly in the early zebrafish embryo. Because Dynamin plays a well-described role in vesicle scission during endocytosis, we predicted that Dynamin might regulate epiboly through participating in bulk removal of the yolk cell membrane ahead of the advancing margin, a proposed part of the epiboly motor. Unexpectedly, we found that Dynamin function was dispensable in the yolk cell and instead, it was required to maintain the epithelial integrity of the EVL during epiboly. Here, we present a model describing the maintenance of EVL integrity, which is required for the proper generation and transmission of tension during epiboly. Furthermore, we discuss the role of Dynamin-mediated regulation of ezrin-radixin-moesin (ERM) family proteins in the maintenance of epithelial integrity.

Genes / Markers
Figures
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping