PUBLICATION

Fish in chips: an automated microfluidic device to study drug dynamics in vivo using zebrafish embryos

Authors
Zheng, C., Zhou, H., Liu, X., Pang, Y., Zhang, B., and Huang, Y.
ID
ZDB-PUB-140127-5
Date
2014
Source
Chemical communications (Cambridge, England)   50(8): 981-984 (Journal)
Registered Authors
Liu, Xinxing, Zhang, Bo
Keywords
none
MeSH Terms
  • Animals
  • Animals, Genetically Modified/growth & development
  • Automation
  • Embryo, Nonmammalian/drug effects*
  • Embryo, Nonmammalian/metabolism
  • Embryonic Development/drug effects
  • Fish Proteins/metabolism
  • Green Fluorescent Proteins/genetics
  • Green Fluorescent Proteins/metabolism
  • Hedgehog Proteins/metabolism
  • Microfluidic Analytical Techniques/instrumentation
  • Microfluidic Analytical Techniques/methods*
  • Signal Transduction/drug effects
  • Veratrum Alkaloids/chemistry
  • Veratrum Alkaloids/toxicity*
  • Zebrafish/growth & development*
PubMed
24305733 Full text @ Chem. Commun. (Camb.)
Abstract

Interference of the Hedgehog (Hh) signaling pathway by cyclopamine leads to abnormal embryonic development. We monitor this dynamical drug effect in zebrafish embryos with highly precise microenvironment control using an integrated microfluidic device. This chip-based platform, which is programmable and automated, greatly facilitates the accuracy and reproducibility of the in vivo assays.

Genes / Markers
Figures
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping