PUBLICATION

Differential, dominant activation and inhibition of Notch signalling and APP cleavage by truncations of PSEN1 in human disease

Authors
Newman, M., Wilson, L., Verdile, G., Lim, A., Khan, I., Moussavi Nik, S.H., Pursglove, S., Chapman, G., Martins, R.N., and Lardelli, M.
ID
ZDB-PUB-131112-8
Date
2014
Source
Human molecular genetics   23(3): 602-17 (Journal)
Registered Authors
Lardelli, Michael, Newman, Morgan
Keywords
none
MeSH Terms
  • Amino Acid Sequence
  • Amyloid beta-Protein Precursor/metabolism*
  • Amyloidogenic Proteins/genetics
  • Amyloidogenic Proteins/metabolism
  • Animals
  • Base Sequence
  • Basic Helix-Loop-Helix Transcription Factors/genetics
  • Basic Helix-Loop-Helix Transcription Factors/metabolism
  • Embryo, Nonmammalian
  • Exons
  • HEK293 Cells
  • Hidradenitis Suppurativa/genetics
  • Humans
  • Intracellular Membranes/metabolism
  • Mice
  • Molecular Sequence Data
  • Molecular Weight
  • Mutation
  • Nerve Tissue Proteins/genetics
  • Nerve Tissue Proteins/metabolism
  • Pick Disease of the Brain/genetics
  • Presenilin-1/genetics
  • Presenilin-1/metabolism*
  • Presenilin-2/genetics
  • Presenilin-2/metabolism
  • Receptors, Notch/genetics
  • Receptors, Notch/metabolism*
  • Signal Transduction
  • Zebrafish/embryology
  • Zebrafish/metabolism
  • Zebrafish Proteins/genetics
  • Zebrafish Proteins/metabolism
PubMed
24101600 Full text @ Hum. Mol. Genet.
Abstract

PRESENILIN1 (PSEN1) is the major locus for mutations causing familial Alzheimer's disease (FAD) and is also mutated in Pick disease of brain, familial acne inversa and dilated cardiomyopathy. It is a critical facilitator of Notch signalling and many other signalling pathways and protein cleavage events including production of the Amyloidβ (Aβ) peptide from the AMYLOID BETA A4 PRECURSOR PROTEIN (APP). We previously reported that interference with splicing of transcripts of the zebrafish orthologue of PSEN1 creates dominant negative effects on Notch signalling. Here, we extend this work to show that various truncations of human PSEN1 (or zebrafish Psen1) protein have starkly differential effects on Notch signalling and cleavage of zebrafish Appa (a paralogue of human APP). Different truncations can suppress or stimulate Notch signalling but not Appa cleavage and vice versa. The G183V mutation possibly causing Pick disease causes production of aberrant transcripts truncating the open reading frame after exon 5 sequence. We show that the truncated protein potentially translated from these transcripts avidly incorporates into very stable Psen1-dependent higher molecular weight complexes and suppresses cleavage of Appa but not Notch signalling. In contrast, the truncated protein potentially produced by the P242LfsX11 acne inversa mutation has no effect on Appa cleavage but, unexpectedly, enhances Notch signalling. Our results suggest novel hypotheses for the pathological mechanisms underlying these diseases and illustrate the importance of investigating the function of dominant mutations at physiologically relevant expression levels and in the normally heterozygous state in which they cause human disease rather than in isolation from healthy alleles.

Genes / Markers
Figures
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping