PUBLICATION

Bis-(2-ethylexhyl) phthalate impairs spermatogenesis in zebrafish (Danio rerio)

Authors
Corradetti, B., Stronati, A., Tosti, L., Manicardi, G., Carnevali, O., and Bizzaro, D.
ID
ZDB-PUB-130911-5
Date
2013
Source
Reproductive Biology   13(3): 195-202 (Journal)
Registered Authors
Carnevali, Oliana
Keywords
endocrine disruptors, DEHP, Danio rerio, spermatogenesis, zebrafish
MeSH Terms
  • Animals
  • DNA Fragmentation/drug effects
  • Diethylhexyl Phthalate/toxicity*
  • Ethinyl Estradiol/pharmacology
  • Male
  • Reproduction/drug effects
  • Spermatogenesis/drug effects*
  • Zebrafish
PubMed
24011190 Full text @ Reprod. Biol.
Abstract

Bis-(2-ethylhexyl) phthalate (DEHP) is a widely used industrial additive for increasing plastic flexibility. Its metabolites are known to exert toxic effects on reproduction and development of mammals. The aim of this study was to evaluate the effects of environmentally relevant concentrations of DEHP (0.2 and 20 μg/L) on the reproductive biology of adult male zebrafish (Danio rerio). The effects of DEHP and 17β-ethynylestradiol (a positive control) were determined after one or three weeks of exposure by TUNEL assay, histomorphometric analysis and evaluation of reproductive performance. DEHP impaired reproduction in zebrafish by inducing a mitotic arrest during spermatogenesis, increasing DNA fragmentation in sperm cells and markedly reducing embryo production (up to 90%). In conclusion, relatively short-term exposure to environmentally relevant concentrations of DEHP is able to alter spermatogenesis and affect reproduction in zebrafish.

Genes / Markers
Figures
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping