PUBLICATION

Cloning and promoter analysis of gonadotrophin-releasing hormone a in the orange-spotted grouper (Epinephelus coioides)

Authors
Chen, J., Luo, Y.S., Zhu, Z.Y., and Hu, W.
ID
ZDB-PUB-130726-5
Date
2013
Source
Yi chuan = Hereditas   35(7): 896-902 (Journal)
Registered Authors
Hu, Wei
Keywords
none
MeSH Terms
  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Cloning, Molecular*
  • Fish Proteins/chemistry
  • Fish Proteins/genetics*
  • Fish Proteins/metabolism
  • Gonadotropin-Releasing Hormone/chemistry
  • Gonadotropin-Releasing Hormone/genetics*
  • Gonadotropin-Releasing Hormone/metabolism
  • Humans
  • Molecular Sequence Data
  • Perciformes/classification
  • Perciformes/genetics*
  • Perciformes/metabolism
  • Promoter Regions, Genetic*
  • Sequence Alignment
PubMed
23853361 Full text @ Yi Chuan
Abstract
Gonadotrophin-releasing hormone (GnRH) is a key regulator of reproduction in all vertebrates. We first cloned the cDNA and genomic DNA sequences coding for GnRHa gene in the orange-spotted grouper (Epinephelis coioides), an economically important marine fish, and then cloned its promoter sequence. The region responsible for the cell-specific expression of GnRHa was located between -2005 bp to -956 bp from the translation start site. GnRHa promoter driven EGFP expression in transgenic zebrafish showed that GnRHa-positive neurons were primarily located in the midbrain and in the eyes. Our results provide an improved understanding of the regulatory mechanism and function of GnRHa of E. coioides.
Errata / Notes
Article is in Chinese.
Genes / Markers
Figures
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping