PUBLICATION
Construction and assessment of heart-specific green fluorescence zebrafish line
- Authors
- Peng, X.Y., Chen, T.F., Huang, T., Jiang, Z.G., Wu, X.S., and Deng, Y.
- ID
- ZDB-PUB-130610-31
- Date
- 2013
- Source
- Yi chuan = Hereditas 35(4): 511-518 (Journal)
- Registered Authors
- Deng, Yun, Wu, Xiushan
- Keywords
- none
- MeSH Terms
-
- Animals
- Animals, Genetically Modified
- Gene Expression Regulation
- Genetic Vectors/genetics
- Green Fluorescent Proteins/genetics*
- Heart/physiology
- Myocardium/metabolism*
- Myosin Light Chains/genetics
- Organ Specificity
- Zebrafish/genetics*
- Zebrafish/physiology
- Zebrafish Proteins/genetics
- PubMed
- 23659942 Full text @ Yi Chuan
Citation
Peng, X.Y., Chen, T.F., Huang, T., Jiang, Z.G., Wu, X.S., and Deng, Y. (2013) Construction and assessment of heart-specific green fluorescence zebrafish line. Yi chuan = Hereditas. 35(4):511-518.
Abstract
Using the promoter for cardiac myosin light chain 2 (cmlc2) gene, an expression vector pTol2-cmlc2-IRES- EGFP for making heart-specific expression of exogenous gene in transgenic zebrafish was generated previously. Here, we reported the construction of a transgenic zebrafish line which stably expresses EGFP using this vector, and the effects of EGFP on the heart development and cardiac function of this transgenic zebrafish line were preliminarily analyzed. The results showed that the green fluorescence signal of cmlc2:EGFP line under fluorescence microscopy specifically expressed in heart and faithfully recapitulated both the spatial and temporal expression patterns of endogenous cmlc2 gene revealed by in situ hybridization in the early developmental stages. The cardiac morphology and development of this transgenic zebrafish line remained to be normal. Furthermore, the heart morphology and physiological function of this transgenic line have been analyzed using M-mode analysis. The results showed that there was no significant difference between the cmlc2:EGFP and the wild type lines with respect to heart period, heart rate, diastolic surface area and systolic surface area, and fractional area change. No tachyarrhythmia was observed in the embryos from either line. Thus, the excessive expression of EGFP in this transgenic line seemed to exert no detrimental effects on the function and development of zebrafish hearts during early stages. Our study laid a foundation for the construction of exogenous gene transgenic line using pTol2-cmlc2-IRES-EGFP vector to study the function of genes that expressed in heart.
Errata / Notes
Article is in Chinese.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping