PUBLICATION

Effects of Maternal Cadmium Exposure on Female Reproductive Functions, Gamete Quality, and Offspring Development in Zebrafish (Danio rerio)

Authors
Wu, S.M., Tsai, P.J., Chou, M.Y., and Wang, W.D.
ID
ZDB-PUB-130607-5
Date
2013
Source
Archives of environmental contamination and toxicology   65(3): 521-36 (Journal)
Registered Authors
Chou, Ming-Yi, Wang, Wen-Der
Keywords
none
Datasets
GEO:GSE20926
MeSH Terms
  • Animals
  • Cadmium/toxicity*
  • Embryo, Nonmammalian/drug effects
  • Embryo, Nonmammalian/embryology
  • Female
  • Fish Proteins/genetics
  • Fish Proteins/metabolism
  • Gene Expression Regulation/drug effects
  • Larva/drug effects
  • Larva/growth & development
  • Maternal Exposure
  • Oligonucleotide Array Sequence Analysis
  • Ovary/drug effects
  • Ovary/pathology
  • Ovum/cytology
  • Ovum/drug effects
  • Real-Time Polymerase Chain Reaction
  • Reproduction/drug effects*
  • Spectrophotometry, Atomic
  • Water Pollutants, Chemical/toxicity*
  • Zebrafish/growth & development
  • Zebrafish/physiology*
PubMed
23644582 Full text @ Arch. Environ. Contam. Toxicol.
CTD
23644582
Abstract

Impacts of maternal Cd2+ exposure on female zebrafish (Danio rerio) were observed in females as well as their offspring. In females, Cd disturbed fecundity and other reproductive functions. In their offspring, it retarded gamete development and growth and influenced gene expression. There was a positive relationship between Cd2+ contents in ovaries of females and treatment doses of 0–8.9 μM of Cd2+. The mating rate decreased by 60 % when females were exposed to 8.9–35.6 μM of Cd2+ for 72 h compared with the control group. It was observed that growth is delayed by one somite stage in maternal-Cd2+ embryos compared with control embryos, which grew at the sixth-somite stage. The ceratohyal angles of larvae of Cd-exposed adults (maternal Cd2+) at 72 h postfertilization (hpf) appeared to have a positive response after doses of maternal Cd. In addition, approximately 30 % of 96-hpf larvae that were treated with a dose of 35.6 μM of maternal Cd2+ appeared to have pericardial edema. At the 5-hpf stage of maternal Cd2+ exposure, embryos showed 33 and 37 target genes, respectively, that were significantly downregulated and upregulated as shown by cDNA microarray analysis. A major effect of maternal Cd2+ exposure on zebrafish embryo genes is that 18.9 % of transcription functions were upregulated. In addition, 33.3 % of transcripts relative to the function of protein biosynthesis were downregulated. These results showed that maternal Cd2+ exposure influenced the reproduction ability of females and also caused their embryos to develop with abnormal gene expression.

Genes / Markers
Figures
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping