PUBLICATION

Introduction of Aequorin into Zebrafish Embryos for Recording Ca(2+) Signaling during the First 48 h of Development

Authors
Webb, S.E., Chan, C.M., and Miller, A.L.
ID
ZDB-PUB-130605-8
Date
2013
Source
Cold Spring Harbor protocols   2013(5): 383-6 (Review)
Registered Authors
Miller, Andrew L., Webb, Sarah E.
Keywords
none
MeSH Terms
  • Aequorin/genetics*
  • Aequorin/metabolism
  • Animals
  • Calcium/metabolism*
  • Developmental Biology/methods*
  • Embryo, Nonmammalian
  • Genes, Reporter*
  • Luminescent Measurements
  • Signal Transduction*
  • Staining and Labeling/methods
  • Zebrafish/embryology*
  • Zebrafish/genetics
  • Zebrafish/growth & development
  • Zebrafish/physiology
PubMed
23637358 Full text @ Cold Spring Harb. Protoc.
Abstract

Ca2+ signals, whether transient pulses, propagating waves, or long-duration, steady gradients, are generally considered to play an important role in the pattern-forming events that occur during vertebrate development. One vertebrate that has long been a favorite of embryologists because of its ex utero development and the optical clarity of its embryos is the zebrafish, Danio rerio. Using the bioluminescent Ca2+ reporter aequorin, distinct Ca2+ signals have been reported for at least the first 48 h of zebrafish development, with signals becoming progressively more complex as the embryo develops. Here we provide a general introduction to aequorin and its use in monitoring Ca2+ signals and discuss methods for introducing aequorin into zebrafish embryos.

Genes / Markers
Figures
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping