PUBLICATION

Methods for Culturing Saltwater Rotifers (Brachionus plicatilis) for Rearing Larval Zebrafish

Authors
Lawrence, C., Sanders, E., and Henry, E.
ID
ZDB-PUB-120907-13
Date
2012
Source
Zebrafish   9(3): 140-146 (Journal)
Registered Authors
Lawrence, Christian
Keywords
none
MeSH Terms
  • Animal Feed
  • Animal Husbandry/economics
  • Animal Husbandry/instrumentation
  • Animal Husbandry/methods*
  • Animals
  • Aquaculture/economics
  • Aquaculture/instrumentation
  • Aquaculture/methods*
  • Larva/growth & development
  • Rotifera/growth & development*
  • Rotifera/physiology
  • Water/chemistry
  • Zebrafish/growth & development
PubMed
22950820 Full text @ Zebrafish
Abstract

The saltwater rotifer, Brachionus plicatilis, is widely used in the aquaculture industry as a prey item for first-feeding fishes due to its ease of culture, small size, rapid reproductive rate, and amenability to enrichment with nutrients. Despite the distinct advantages of this approach, rotifers have only been sporadically utilized for rearing larval zebrafish, primarily because of the common misconception that maintaining cultures of rotifers is difficult and excessively time-consuming. Here we present simple methods for maintaining continuous cultures of rotifers capable of supporting even the very largest zebrafish aquaculture facility, with minimal investments in materials, time, labor, and space. Examples of the methods' application in one large, existing facility is provided, and troubleshooting of common problems is discussed.

Genes / Markers
Figures
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping