PUBLICATION

Methodological approaches to cytochrome p450 profiling in embryos

Authors
Goldstone, J.V., and Stegeman, J.J.
ID
ZDB-PUB-120727-15
Date
2012
Source
Methods in molecular biology (Clifton, N.J.)   889: 265-275 (Chapter)
Registered Authors
Goldstone, Jed, Stegeman, John J.
Keywords
cytochrome P450, CYP, zebrafish, development, microarray, quantitative RT-PCR, qPCR
MeSH Terms
  • Animals
  • Culture Techniques
  • Cytochrome P-450 Enzyme System/genetics*
  • Cytochrome P-450 Enzyme System/metabolism
  • Embryo, Nonmammalian/enzymology*
  • Female
  • Gene Expression
  • Gene Expression Profiling
  • Gene Expression Regulation, Developmental
  • Male
  • Oligonucleotide Array Sequence Analysis
  • RNA, Messenger/genetics
  • RNA, Messenger/isolation & purification
  • Zebrafish/embryology
  • Zebrafish/genetics
PubMed
22669670 Full text @ Meth. Mol. Biol.
Abstract

Cytochrome P450 enzymes (CYPs) are heme thiolate proteins essential for vertebrate development and also play important roles in toxicology as well as normal metabolic function. CYP enzymes catalyze the oxidative biotransformation of many endogenous and exogenous chemicals, including steroids and eicosanoids, and drugs and other xenobiotic toxicants. Many CYPs have known for their potential roles in development, and many chemicals that cause developmental abnormalities are substrates for CYPs. The roles and regulation of most xenobiotic metabolizing CYPs during development are unknown, impeding understanding of mechanisms of developmental toxicity. The zebrafish (Danio rerio) has become one of the premier models in vertebrate developmental biology, in large part because of the logistical advantages of rapid external development, high adult maintenance density, and significant (bio)technological tool availability. The increasing use of zebrafish in drug discovery and mechanistic toxicology demands knowledge of CYP gene regulation and function. Here, we present methods to examine CYP expression during early development in zebrafish, with an emphasis on developmental microarrays.

Genes / Markers
Figures
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping