PUBLICATION
3D adaptive optics in a light sheet microscope
- Authors
- Bourgenot, C., Saunter, C.D., Taylor, J.M., Girkin, J.M., and Love, G.D.
- ID
- ZDB-PUB-120702-31
- Date
- 2012
- Source
- Optics express 20(12): 13252-13261 (Journal)
- Registered Authors
- Taylor, John
- Keywords
- none
- MeSH Terms
-
- Animals
- Calibration
- Green Fluorescent Proteins/metabolism
- Imaging, Three-Dimensional/instrumentation*
- Light*
- Lighting
- Microscopy/instrumentation*
- Optics and Photonics/instrumentation*
- Plastics
- Refractometry
- Zebrafish
- PubMed
- 22714353 Full text @ Opt. Express
Citation
Bourgenot, C., Saunter, C.D., Taylor, J.M., Girkin, J.M., and Love, G.D. (2012) 3D adaptive optics in a light sheet microscope. Optics express. 20(12):13252-13261.
Abstract
We report on a single plane illumination microscope (SPIM) incorporating adaptive optics in the imaging arm. We show how aberrations can occur from the sample mounting tube and quantify the aberrations both experimentally and computationally. A wavefront sensorless approach was taken to imaging a green fluorescent protein (GFP) labelled transgenic zebrafish. We show improvements in image quality whilst recording a 3D “z–stack” and show how the aberrations come from varying depths in the fish.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping