PUBLICATION

Cyclic Caged Morpholinos: Conformationally Gated Probes of Embryonic Gene Function

Authors
Yamazoe, S., Shestopalov, I.A., Provost, E., Leach, S.D., and Chen, J.K.
ID
ZDB-PUB-120630-3
Date
2012
Source
Angewandte Chemie (International ed. in English)   51(28): 6908-6911 (Journal)
Registered Authors
Chen, James K., Leach, Steven D., Shestopalov, Ilya
Keywords
antisense oligonucleotides, developmental biology, gene expression, oligonucleotides, zebrafish
MeSH Terms
  • Animals
  • Cell Differentiation
  • Embryo, Nonmammalian/cytology
  • Embryo, Nonmammalian/metabolism*
  • Gene Expression*
  • Gene Expression Regulation, Developmental*
  • Green Fluorescent Proteins/genetics
  • Green Fluorescent Proteins/metabolism
  • Larva/cytology
  • Larva/metabolism*
  • Morpholinos/pharmacology*
  • Oligonucleotides/pharmacology*
  • Organogenesis
  • Pancreas/embryology
  • Pancreas/metabolism*
  • Zebrafish/embryology
  • Zebrafish/genetics
  • Zebrafish/metabolism
PubMed
22689470 Full text @ Angew. Chem. Int. Ed. Engl.
Abstract

Feeling a bit cagey: Morpholino-based antisense reagents have been caged through oligonucleotide cyclization (see scheme), enabling photocontrol of gene expression in zebrafish embryos and larvae. Using these reagents, the timing of exocrine cell fate commitment in the developing pancreas has been examined.

Genes / Markers
Figures
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping