PUBLICATION

Multiview deblurring for 3-D images from light-sheet-based fluorescence microscopy

Authors
Temerinac-Ott, M., Ronneberger, O., Ochs, P., Driever, W., Brox, T., and Burkhardt, H.
ID
ZDB-PUB-120628-1
Date
2012
Source
IEEE transactions on image processing : a publication of the IEEE Signal Processing Society   21(4): 1863-1873 (Journal)
Registered Authors
Driever, Wolfgang
Keywords
none
MeSH Terms
  • Algorithms*
  • Artificial Intelligence
  • Image Enhancement/methods*
  • Image Interpretation, Computer-Assisted/methods*
  • Imaging, Three-Dimensional/methods*
  • Microscopy, Fluorescence/methods*
  • Pattern Recognition, Automated/methods*
  • Reproducibility of Results
  • Sensitivity and Specificity
PubMed
22203719 Full text @ IEEE Trans. Image Process.
Abstract

We propose an algorithm for 3-D multiview deblurring using spatially variant point spread functions (PSFs). The algorithm is applied to multiview reconstruction of volumetric microscopy images. It includes registration and estimation of the PSFs using irregularly placed point markers (beads). We formulate multiview deblurring as an energy minimization problem subject to L1-regularization. Optimization is based on the regularized Lucy-Richardson algorithm, which we extend to deal with our more general model. The model parameters are chosen in a profound way by optimizing them on a realistic training set. We quantitatively and qualitatively compare with existing methods and show that our method provides better signal-to-noise ratio and increases the resolution of the reconstructed images.

Genes / Markers
Figures
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping