PUBLICATION

High dynamic range optical projection tomography (HDR-OPT)

Authors
Fei, P., Yu, Z., Wang, X., Lu, P.J., Fu, Y., He, Z., Xiong, J., and Huang, Y.
ID
ZDB-PUB-120424-1
Date
2012
Source
Optics express   20(8): 8824-8836 (Journal)
Registered Authors
Xiong, Jing-Wei
Keywords
none
MeSH Terms
  • Algorithms
  • Animals
  • Blood Vessels/embryology
  • Cartilage/embryology
  • Image Processing, Computer-Assisted/statistics & numerical data
  • Imaging, Three-Dimensional/statistics & numerical data
  • Optical Phenomena
  • Staining and Labeling
  • Tomography, Optical/instrumentation
  • Tomography, Optical/methods*
  • Tomography, Optical/statistics & numerical data
  • Zebrafish/embryology
PubMed
22513593 Full text @ Opt. Express
Abstract

Traditional optical projection tomography (OPT) acquires a single image at each rotation angle, thereby suffering from limitations in CCD dynamic range; this conventional usage cannot resolve features in samples with highly heterogeneous absorption, such as in small animals with organs of varying size. We present a novel technique, applying multiple-exposure high dynamic range (HDR) imaging to OPT, and demonstrate its ability to resolve fine details in zebrafish embryos, without complicated chemical clearing. We implement the tomographic reconstruction algorithm on the GPU, yielding a performance increase of two orders of magnitude. These features give our method potential application in high-throughput, high-resolution in vivo 3D imaging.

Genes / Markers
Figures
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping