PUBLICATION

Sex differences in transcriptional expression of FABPs in zebrafish liver after chronic perfluorononanoic acid exposure

Authors
Zhang, W., Zhang, Y., Zhang, H., Wang, J., Cui, R., and Dai, J.
ID
ZDB-PUB-120417-2
Date
2012
Source
Environmental science & technology   46(9): 5175-5182 (Journal)
Registered Authors
Zhang, Wei
Keywords
none
MeSH Terms
  • Animals
  • CCAAT-Enhancer-Binding Proteins/metabolism
  • Cholesterol/metabolism
  • Environmental Pollutants/administration & dosage*
  • Fatty Acid-Binding Proteins/metabolism*
  • Female
  • Fluorocarbons/administration & dosage*
  • Gene Expression/drug effects
  • Liver/drug effects*
  • Liver/metabolism
  • Male
  • Peroxisome Proliferator-Activated Receptors/metabolism
  • Sex Factors
  • Triglycerides/metabolism
  • Zebrafish/metabolism*
PubMed
22500729 Full text @ Env. Sci. Tech.
Abstract

Perfluorononanoic acid (PFNA), a nine carbon backbone of perfluorinated acids (PFAAs), has wide production applications and is found in environmental matrices as a contaminant. To understand the adverse effects of PFNA, adult male and female zebrafish were exposed to differing PFNA dosages (0, 0.01, 0.1, and 1.0 mg/L) for 180 days using a flow-through exposure system. Results showed body weight, body length, and hepatosomatic index (HSI) decreased in both sexes. The HPLC-MS/MS analysis found that PFNA concentrations were higher in male livers than in female livers with increasing significance in a dose-dependent manner. Total cholesterol levels increased in the livers of both sexes, while triglyceride (TG) levels increased in males and decreased in females. With the exception of FABP1b, the transcriptional expression levels of fatty acid binding proteins (FABPs) were up-regulated in males and down-regulated in females. A similar trend between sexes occurred for peroxisome proliferator-activated receptors (PPARs) and Ccaat-enhancer-binding proteins (C/EBPs), which may be the upstream regulatory elements of FABPs. The results indicated that PFNA exposure caused opposite adverse effects on liver TG levels between the sexes in zebrafish, possibly due to the opposite expression of FABPs and its upstream genes.

Genes / Markers
Figures
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping