PUBLICATION

Using the zebrafish photomotor response for psychotropic drug screening

Authors
Kokel, D., and Peterson, R.T.
ID
ZDB-PUB-111012-26
Date
2011
Source
Methods in cell biology   105: 517-524 (Chapter)
Registered Authors
Peterson, Randall
Keywords
adaptation, barcoding, multiwell, photomotor, phychotropic, stimulus
MeSH Terms
  • Algorithms
  • Animals
  • Antipsychotic Agents/pharmacology*
  • Automation, Laboratory
  • Behavior, Animal/drug effects
  • Behavior, Animal/physiology
  • Behavior, Animal/radiation effects
  • Dark Adaptation
  • Drug Discovery/methods*
  • Drug Evaluation, Preclinical/methods*
  • Embryo, Nonmammalian/drug effects
  • Embryo, Nonmammalian/physiology*
  • Embryo, Nonmammalian/radiation effects
  • High-Throughput Screening Assays*
  • Humans
  • Infrared Rays
  • Motor Activity/drug effects
  • Motor Activity/physiology
  • Motor Activity/radiation effects
  • Phenotype
  • Psychotic Disorders/drug therapy
  • Research Design
  • Small Molecule Libraries/pharmacology*
  • Video Recording/instrumentation
  • Video Recording/methods
  • Zebrafish/embryology
  • Zebrafish/physiology*
PubMed
21951545 Full text @ Meth. Cell. Biol.
Abstract
Because psychotropic drugs affect behavior, we can use changes in behavior to discover psychotropic drugs. The original prototypes of most neuroactive medicines were discovered in humans, rodents and other model organisms. Most of these discoveries were made by chance, but the process of behavior based drug discovery can be made more systematic and efficient. Fully automated platforms for analyzing the behavior of embryonic zebrafish capture digital video recordings of animals in each individual well of a 96-well plate before, during, and after a series of stimuli. To analyze systematically the thousands of behavioral recordings obtained from a large-scale chemical screen, we transform these behavioral recordings into numerical barcodes, providing a concise and interpretable summary of the observed phenotypes in each well. Systems-level analysis of these behavioral phenotypes generate testable hypotheses about the molecular mechanisms of poorly understood drugs and behaviors. By combining the in vivo relevance of behavior-based phenotyping with the scale and automation of modern drug screening technologies, systematic behavioral barcoding represents a means of discovering psychotropic drugs and provides a powerful, systematic approach for unraveling the complexities of vertebrate behavior.
Genes / Markers
Figures
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping