PUBLICATION

Tol2-mediated Transgenesis, Gene Trapping, Enhancer Trapping, and the Gal4-UAS System

Authors
Abe, G., Suster, M.L., and Kawakami, K.
ID
ZDB-PUB-110921-4
Date
2011
Source
Methods in cell biology   104: 23-49 (Chapter)
Registered Authors
Kawakami, Koichi, Suster, Maximiliano
Keywords
enhancer, gene trapping, germline, microinjection, transgenesis, transposable
MeSH Terms
  • Animals
  • Animals, Genetically Modified
  • Blotting, Southern/methods
  • Chromosomes, Artificial, Bacterial
  • Cloning, Molecular/methods*
  • DNA Transposable Elements*
  • Genes, Reporter
  • Green Fluorescent Proteins/biosynthesis
  • Green Fluorescent Proteins/genetics
  • Microinjections/methods
  • Mutagenesis, Insertional/methods*
  • Recombination, Genetic
  • Transfection/methods
  • Zebrafish/genetics*
PubMed
21924155 Full text @ Meth. Cell. Biol.
Abstract
The Tol2 transposable element was originally found in the genome of the Japanese medaka fish (Oryzias latipes). Tol2 contains a gene encoding an active transposase that can catalyze DNA transposition in vertebrate cells. In zebrafish, Tol2 generates genomic integrations in the germ cells very efficiently. By using the Tol2 transposition system, we have developed important genetic methods including transgenesis, gene trapping, enhancer trapping, and the Gal4-UAS system in zebrafish. In this chapter, we describe how these methods can be performed.
Genes / Markers
Figures
Expression
Phenotype
Mutation and Transgenics
Human Disease / Model Data
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping
Errata and Notes