PUBLICATION
Tol2-mediated Transgenesis, Gene Trapping, Enhancer Trapping, and the Gal4-UAS System
- Authors
- Abe, G., Suster, M.L., and Kawakami, K.
- ID
- ZDB-PUB-110921-4
- Date
- 2011
- Source
- Methods in cell biology 104: 23-49 (Chapter)
- Registered Authors
- Kawakami, Koichi, Suster, Maximiliano
- Keywords
- enhancer, gene trapping, germline, microinjection, transgenesis, transposable
- MeSH Terms
-
- Animals
- Animals, Genetically Modified
- Blotting, Southern/methods
- Chromosomes, Artificial, Bacterial
- Cloning, Molecular/methods*
- DNA Transposable Elements*
- Genes, Reporter
- Green Fluorescent Proteins/biosynthesis
- Green Fluorescent Proteins/genetics
- Microinjections/methods
- Mutagenesis, Insertional/methods*
- Recombination, Genetic
- Transfection/methods
- Zebrafish/genetics*
- PubMed
- 21924155 Full text @ Meth. Cell. Biol.
Citation
Abe, G., Suster, M.L., and Kawakami, K. (2011) Tol2-mediated Transgenesis, Gene Trapping, Enhancer Trapping, and the Gal4-UAS System. Methods in cell biology. 104:23-49.
Abstract
The Tol2 transposable element was originally found in the genome of the Japanese medaka fish (Oryzias latipes). Tol2 contains a gene encoding an active transposase that can catalyze DNA transposition in vertebrate cells. In zebrafish, Tol2 generates genomic integrations in the germ cells very efficiently. By using the Tol2 transposition system, we have developed important genetic methods including transgenesis, gene trapping, enhancer trapping, and the Gal4-UAS system in zebrafish. In this chapter, we describe how these methods can be performed.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping