PUBLICATION

Melatonin Induces Follicle Maturation in Danio rerio

Authors
Carnevali, O., Gioacchini, G., Maradonna, F., Olivotto, I., and Migliarini, B.
ID
ZDB-PUB-110613-14
Date
2011
Source
PLoS One   6(5): e19978 (Journal)
Registered Authors
Carnevali, Oliana
Keywords
none
MeSH Terms
  • Animals
  • Brain/drug effects
  • Brain/physiology
  • Female
  • Fertility/drug effects
  • Melatonin/pharmacology*
  • Ovarian Follicle/drug effects*
  • Ovarian Follicle/growth & development*
  • Pituitary Gland/physiology
  • Reproduction/drug effects
  • Zebrafish/growth & development*
  • Zebrafish/physiology
PubMed
21647435 Full text @ PLoS One
CTD
21647435
Abstract

Adult females were daily exposed, via water, to two different doses (100 nM and 1 µM) of melatonin. Melatonin led to an increase of the Gonado Somatic Index (GSI) associated with the increase of eggs production, and the raise of gene and protein levels of vitellogenin (VTG) and estradiol receptor α (ERα) in the liver. The ability of melatonin to increase fecundity was consistent with a significant increase of gene transcription of kiss 1, kiss 2, gnrh3, in the brain, and lh in the pituitary, while in the ovary (in class IIIB follicles), with a significant decrease of two genes codifying for intra-ovarian regulators of premature oocyte maturation, the tgfβ1 and the bmp15. The reduction in the expression of these two genes was concomitant with the increase of lhr and a modulation of mprα and mprβ gene transcription, whose proteins are involved in oocyte maturation. Melatonin also exerted a direct action on follicles as shown by the increase of the oocytes undergoing to germinal vesicle break down (GVBD) and modulated mpr α and β gene expression in the in vitro exposure.

Genes / Markers
Figures
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping