PUBLICATION
In vivo fluorescence lifetime optical projection tomography
- Authors
- McGinty, J., Taylor, H.B., Chen, L., Bugeon, L., Lamb, J.R., Dallman, M.J., and French, P.M.
- ID
- ZDB-PUB-110524-5
- Date
- 2011
- Source
- Biomedical Optics Express 2(5): 1340-1350 (Journal)
- Registered Authors
- Bugeon, Laurence, Dallman, Maggie, Lamb, Jonathan
- Keywords
- none
- MeSH Terms
- none
- PubMed
- 21559145 Full text @ Biomed. Opt. Express
Citation
McGinty, J., Taylor, H.B., Chen, L., Bugeon, L., Lamb, J.R., Dallman, M.J., and French, P.M. (2011) In vivo fluorescence lifetime optical projection tomography. Biomedical Optics Express. 2(5):1340-1350.
Abstract
We demonstrate the application of fluorescence lifetime optical projection tomography (FLIM-OPT) to in vivo imaging of lysC:GFP transgenic zebrafish embryos (Danio rerio). This method has been applied to unambiguously distinguish between the fluorescent protein (GFP) signal in myeloid cells from background autofluorescence based on the fluorescence lifetime. The combination of FLIM, an inherently ratiometric method, in conjunction with OPT results in a quantitative 3-D tomographic technique that could be used as a robust method for in vivo biological and pharmaceutical research, for example as a readout of Förster resonance energy transfer based interactions.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping