PUBLICATION

In vivo fluorescence lifetime optical projection tomography

Authors
McGinty, J., Taylor, H.B., Chen, L., Bugeon, L., Lamb, J.R., Dallman, M.J., and French, P.M.
ID
ZDB-PUB-110524-5
Date
2011
Source
Biomedical Optics Express   2(5): 1340-1350 (Journal)
Registered Authors
Bugeon, Laurence, Dallman, Maggie, Lamb, Jonathan
Keywords
none
MeSH Terms
none
PubMed
21559145 Full text @ Biomed. Opt. Express
Abstract
We demonstrate the application of fluorescence lifetime optical projection tomography (FLIM-OPT) to in vivo imaging of lysC:GFP transgenic zebrafish embryos (Danio rerio). This method has been applied to unambiguously distinguish between the fluorescent protein (GFP) signal in myeloid cells from background autofluorescence based on the fluorescence lifetime. The combination of FLIM, an inherently ratiometric method, in conjunction with OPT results in a quantitative 3-D tomographic technique that could be used as a robust method for in vivo biological and pharmaceutical research, for example as a readout of Förster resonance energy transfer based interactions.
Genes / Markers
Figures
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping