PUBLICATION
A guide to analysis of cardiac phenotypes in the zebrafish embryo
- Authors
- Miura, G.I., and Yelon, D.
- ID
- ZDB-PUB-110523-3
- Date
- 2011
- Source
- Methods in cell biology 101: 161-180 (Chapter)
- Registered Authors
- Yelon, Deborah
- Keywords
- action potential, atrioventricular canal, cardiac function, cardiogenesis, heart size defect, pericardial edema
- MeSH Terms
-
- Animals
- Gene Expression Regulation, Developmental
- Heart/embryology*
- Morphogenesis
- Phenotype
- Zebrafish/embryology*
- Zebrafish/genetics
- PubMed
- 21550443 Full text @ Meth. Cell. Biol.
Citation
Miura, G.I., and Yelon, D. (2011) A guide to analysis of cardiac phenotypes in the zebrafish embryo. Methods in cell biology. 101:161-180.
Abstract
The zebrafish is an ideal model organism for investigating the molecular mechanisms underlying cardiogenesis, due to the powerful combination of optical access to the embryonic heart and plentiful opportunities for genetic analysis. A continually increasing number of studies are uncovering mutations, morpholinos, and small molecules that cause striking cardiac defects and disrupt blood circulation in the zebrafish embryo. Such defects can result from a wide variety of origins including defects in the specification or differentiation of cardiac progenitor cells; errors in the morphogenesis of the heart tube, the cardiac chambers, or the atrioventricular canal or problems with establishing proper cardiac function. An extensive arsenal of techniques is available to distinguish between these possibilities and thereby decipher the roots of cardiac defects. In this chapter, we provide a guide to the experimental strategies that are particularly effective for the characterization of cardiac phenotypes in the zebrafish embryo.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping