PUBLICATION
Time-lapse Live Imaging of Clonally Related Neural Progenitor Cells in the Developing Zebrafish Forebrain
- Authors
- Dong, Z., Wagle, M., and Guo, S.
- ID
- ZDB-PUB-110518-24
- Date
- 2011
- Source
- Journal of visualized experiments : JoVE (50): (Journal)
- Registered Authors
- Dong, Zhiqiang, Guo, Su, Wagle, Mahendra
- Keywords
- none
- MeSH Terms
-
- Animals
- Clone Cells
- Cytological Techniques/methods
- Electroporation/methods
- Female
- Fluorescent Dyes/chemistry
- Male
- Microscopy, Confocal/methods*
- Neural Stem Cells/cytology*
- Prosencephalon/cytology*
- Prosencephalon/embryology
- Zebrafish/embryology*
- PubMed
- 21505407 Full text @ J. Vis. Exp.
Citation
Dong, Z., Wagle, M., and Guo, S. (2011) Time-lapse Live Imaging of Clonally Related Neural Progenitor Cells in the Developing Zebrafish Forebrain. Journal of visualized experiments : JoVE. (50).
Abstract
Precise patterns of division, migration and differentiation of neural progenitor cells are crucial for proper brain development and function(1,2). To understand the behavior of neural progenitor cells in the complex in vivo environment, time-lapse live imaging of neural progenitor cells in an intact brain is critically required. In this video, we exploit the unique features of zebrafish embryos to visualize the development of forebrain neural progenitor cells in vivo. We use electroporation to genetically and sparsely label individual neural progenitor cells. Briefly, DNA constructs coding for fluorescent markers were injected into the forebrain ventricle of 22 hours post fertilization (hpf) zebrafish embryos and electric pulses were delivered immediately. Six hours later, the electroporated zebrafish embryos were mounted with low melting point agarose in glass bottom culture dishes. Fluorescently labeled neural progenitor cells were then imaged for 36hours with fixed intervals under a confocal microscope using water dipping objective lens. The present method provides a way to gain insights into the in vivo development of forebrain neural progenitor cells and can be applied to other parts of the central nervous system of the zebrafish embryo.
Errata / Notes
This is a video journal article. It has an associated PDF file.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping