PUBLICATION

Two-photon-based photoactivation in live zebrafish embryos

Authors
Russek-Blum, N., Nabel-Rosen, H., and Levkowitz, G.
ID
ZDB-PUB-110110-30
Date
2010
Source
Journal of visualized experiments : JoVE   (46): (Journal)
Registered Authors
Levkowitz, Gil, Nabel-Rosen, Helit, Russek-Blum, Niva
Keywords
none
MeSH Terms
  • Animals
  • Fluorescein/chemistry
  • Fluorescent Dyes/chemistry
  • Green Fluorescent Proteins/biosynthesis
  • Green Fluorescent Proteins/chemistry
  • Green Fluorescent Proteins/genetics
  • Microscopy, Fluorescence, Multiphoton/methods*
  • Zebrafish/embryology*
PubMed
21206475 Full text @ J. Vis. Exp.
Abstract
Photoactivation of target compounds in a living organism has proven a valuable approach to investigate various biological processes such as embryonic development, cellular signaling and adult physiology. In this respect, the use of multi-photon microscopy enables quantitative photoactivation of a given light responsive agent in deep tissues at a single cell resolution. As zebrafish embryos are optically transparent, their development can be monitored in vivo. These traits make the zebrafish a perfect model organism for controlling the activity of a variety of chemical agents and proteins by focused light. Here we describe the use of two-photon microscopy to induce the activation of chemically caged fluorescein, which in turn allows us to follow cell's destiny in live zebrafish embryos. We use embryos expressing a live genetic landmark (GFP) to locate and precisely target any cells of interest. This procedure can be similarly used for precise light induced activation of proteins, hormones, small molecules and other caged compounds.
Genes / Markers
Figures
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping