ZFIN ID: ZDB-PUB-101201-29
Chapter 7 - Photoreceptor Structure and Development Analyses using GFP Transgenes
Perkins, B.D., and Fadool, J.M.
Date: 2010
Source: Methods in cell biology   100: 205-218 (Chapter)
Registered Authors: Fadool, James M., Perkins, Brian
Keywords: none
MeSH Terms:
  • Animals
  • Animals, Genetically Modified
  • Gene Transfer Techniques*
  • Green Fluorescent Proteins/genetics
  • Green Fluorescent Proteins/metabolism
  • Photoreceptor Cells/cytology*
  • Photoreceptor Cells/metabolism
  • Retina/cytology*
  • Retina/metabolism
  • Zebrafish*
PubMed: 21111218 Full text @ Meth. Cell. Biol.
In recent years, studies of zebrafish rod and cone photoreceptors have yielded novel insights into the differentiation of distinct photoreceptor cell types and the mechanisms guiding photoreceptor regeneration following cell death, and they have provided models of human retinal degeneration. These studies were facilitated by the use of transgenic zebrafish expressing fluorescent reporter genes under the control of various cell-specific promoters. Improvements in transgenesis techniques (e.g., Tol2 transposition), the availability of numerous fluorescent reporter genes with different localization properties, and the ability to generate transgenes via recombineering (e.g., Gateway technology) have enabled researchers to quickly develop transgenic lines that improve our understanding of the causes of human blindness and ways to mitigate its effects.