PUBLICATION
A hyperactive sleeping beauty transposase enhances transgenesis in zebrafish embryos
- Authors
- Newman, M., and Lardelli, M.
- ID
- ZDB-PUB-101108-34
- Date
- 2010
- Source
- BMC research notes 3: 282 (Journal)
- Registered Authors
- Lardelli, Michael, Newman, Morgan
- Keywords
- none
- MeSH Terms
- none
- PubMed
- 21050486 Full text @ BMC Res. Notes
Citation
Newman, M., and Lardelli, M. (2010) A hyperactive sleeping beauty transposase enhances transgenesis in zebrafish embryos. BMC research notes. 3:282.
Abstract
BACKGROUND: Transposons are useful molecular tools for transgenesis. The 'sleeping beauty' transposon is a synthetic member of the Tc1/mariner transposon family. Davidson et al. (2003) previously described a vector for zebrafish transgenesis consisting of the inverted repeats of 'sleeping beauty' flanking the gene to be transposed. Subsequently, there have been attempts to enhance the transpositional activity of 'sleeping beauty' by increasing the activity of its transposase. Recently, Mates et al. (2009) generated a hyperactive transposase giving a 100-fold increased transposition rate in mouse embryos. Findings: The aim of this experiment was to determine whether this novel hyperactive transposase enhances transgenesis in zebrafish embryos. Using our previously characterised mitfa-amyloidbeta-GFP transgene, we observed an eight-fold enhancement in transient transgenesis following detection of transgene expression in melanophores by whole mount in-situ hybridisation. However, high rates of defective embryogenesis were also observed. Conclusion: The novel hyperactive 'sleeping beauty' transposase enhances the rate of transgenesis in zebrafish embryos.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping