PUBLICATION

Incorporating Zebrafish Omics into Chemical Biology and Toxicology

Authors
Sukardi, H., Ung, C.Y., Gong, Z., and Lam, S.H.
ID
ZDB-PUB-100420-18
Date
2010
Source
Zebrafish   7(1): 41-52 (Review)
Registered Authors
Gong, Zhiyuan, Lam, Siew Hong
Keywords
none
MeSH Terms
  • Animals
  • Drug Evaluation, Preclinical/methods*
  • Gene Expression Profiling
  • Genetic Testing
  • Genomics/methods*
  • Humans
  • Zebrafish*/genetics
  • Zebrafish*/metabolism
PubMed
20384484 Full text @ Zebrafish
Abstract
In this communication, we describe the general aspects of omics approaches for analyses of transcriptome, proteome, and metabolome, and how they can be strategically incorporated into chemical screening and perturbation studies using the zebrafish system. Pharmacological efficacy and selectivity of chemicals can be evaluated based on chemical-induced phenotypic effects; however, phenotypic observation has limitations in identifying mechanistic action of chemicals. We suggest adapting gene-expression-based high-throughput screening as a complementary strategy to zebrafish-phenotype-based screening for mechanistic insights about the mode of action and toxicity of a chemical, large-scale predictive applications and comparative analysis of chemical-induced omics signatures, which are useful to identify conserved biological responses, signaling pathways, and biomarkers. The potential mechanistic, predictive, and comparative applications of omics approaches can be implemented in the zebrafish system. Examples of these using the omics approaches in zebrafish, including data of ours and others, are presented and discussed. Omics also facilitates the translatability of zebrafish studies across species through comparison of conserved chemical-induced responses. This review is intended to update interested readers with the current omics approaches that have been applied in chemical studies on zebrafish and their potential in enhancing discovery in chemical biology.
Genes / Markers
Figures
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping