PUBLICATION

Culturing and Transfecting Zebrafish PAC2 Fibroblast Cells

Authors
Senghaas, N., and Köster, R.W.
ID
ZDB-PUB-100223-4
Date
2009
Source
CSH protocols   2009(6): pdb.prot5235 (Journal)
Registered Authors
Köster, Reinhard W., Senghaas, Niklas
Keywords
none
MeSH Terms
  • Animals
  • Cell Culture Techniques/methods*
  • Cell Line
  • Cell Survival
  • Cryopreservation
  • Fibroblasts/cytology*
  • Immunohistochemistry
  • Tissue Fixation
  • Transfection/methods*
  • Zebrafish/metabolism*
  • Zebrafish Proteins/isolation & purification
PubMed
20147193 Full text @ CSH Protoc.
Abstract
INTRODUCTION The use of zebrafish (Danio rerio) as a model organism has seen rapid growth in recent years. Numerous new techniques have emerged for zebrafish, and many protocols used in other model organisms have been adapted to fit the special needs of this species. Zebrafish cell lines, however, have not been studied to a great extent, and their applications remain limited. The PAC2 fibroblast line, isolated from 24-h post-fertilization zebrafish embryos, is one of the few available lines. Here, we provide a basic set of methods for maintenance and handling of PAC2 cells, as well as general procedures for transfection, immunocytochemistry, and protein extraction.
Genes / Markers
Figures
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping