PUBLICATION
Cloning of common carp SOCS-3 gene and its expression during embryogenesis, GH-transgene and viral infection
- Authors
- Xiao, Z.G., Liu, H., Fu, J.P., Hu, W., Wang, Y.P., and Guo, Q.L.
- ID
- ZDB-PUB-100112-1
- Date
- 2010
- Source
- Fish & shellfish immunology 28(2): 362-371 (Journal)
- Registered Authors
- Hu, Wei
- Keywords
- SOCS-3, Common carp, Embryogenesis, GH-transgenic carp, Spring viraemia of carp virus (SVCV)
- MeSH Terms
-
- Amino Acid Sequence
- Animals
- Animals, Genetically Modified
- Base Sequence
- Carps/embryology
- Carps/genetics*
- Carps/immunology*
- Carps/virology
- Cloning, Molecular
- Embryonic Development/immunology
- Fish Diseases/immunology*
- Gene Expression Profiling
- Gene Expression Regulation*
- Growth Hormone/genetics
- Molecular Sequence Data
- Phylogeny
- Rhabdoviridae Infections/immunology
- Rhabdoviridae Infections/veterinary*
- Sequence Alignment
- Sequence Homology, Amino Acid
- Suppressor of Cytokine Signaling Proteins/genetics*
- Suppressor of Cytokine Signaling Proteins/immunology*
- Vesiculovirus/immunology
- PubMed
- 20025976 Full text @ Fish Shellfish Immunol.
Citation
Xiao, Z.G., Liu, H., Fu, J.P., Hu, W., Wang, Y.P., and Guo, Q.L. (2010) Cloning of common carp SOCS-3 gene and its expression during embryogenesis, GH-transgene and viral infection. Fish & shellfish immunology. 28(2):362-371.
Abstract
As a member of a newly discovered protein family, the suppressor of cytokine signaling 3 (SOCS-3) has been shown to regulate the responses of many immune cytokines in a negative auto-regulatory manner. The full-length cDNA of common carp SOCS-3 was 1603 bp and contained a 630 bp open reading frame (ORF) coding for a protein of 209 amino acids. Carp SOCS-3 molecule was well conserved especially in the SRC homology 2 (SH2) and the SOCS box. The kinase inhibitory region (KIR) and ESS domains, upstream of the SH2 domain, were conserved in carp SOCS-3s, except for a specific insertion (PHRYK) in the KIR domain at the N-terminal region. Three conserved cytokine (Cys-102, 124 and 193) residues, and one additional cysteine (Cys-168) residue, were also found in carp SOCS-3. The 2015 bp genomic DNA of carp SOCS-3 contained two exons and one intron. Phylogenetic analysis showed that carp SOCS-3 sequence grouped with other known fish SOCS-3 sequences with zebrafish SOCS-3 as the closest neighbor. RT-PCR analysis showed that carp SOCS-3 was initially expressed at 4 h pf (post-fertilization) and gradually increased up to 4 w pf during embryogenesis. By RT-qPCR analysis, carp SOCS-3 gene was predominantly detected in gill, head kidney, thymus and skin, followed by spleen and peripheral blood, lower expression level was detected in kidney, intestine, liver and muscle; the SOCS-3 transcript was significantly increased in thymus, head kidney, spleen and intestine of GH (growth hormone)-transgenic carp; after SVCV (spring viraemia of carp virus) infection, the carp SOCS-3 transcript was significantly up-regulated in gill, intestine, thymus, spleen, head kidney and kidney tissues in a time-dependent manner. These results suggest that teleost SOCS-3 may play an active role in the modulation of viral-induced innate immune response and in preventing the overaction of some cytokines with viral stimulation.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping