PUBLICATION

Cell tracking in fluorescence images of embryogenesis processes with morphological reconstruction by 4D-tubular structuring elements

Authors
Pastor, D., Luengo-Oroz, M.A., Lombardot, B., Gonzalvez, I., Duloquin, L., Savy, T., Bourgine, P., Peyrieras, N., and Santos, A.
ID
ZDB-PUB-091215-46
Date
2009
Source
Conference proceedings : ... Annual International Conference of the IEEE Engineering in Medicine and Biology Society. IEEE Engineering in Medicine and Biology Society. Annual Conference   1: 970-973 (Journal)
Registered Authors
Duloquin, Louise, Savy, Thierry
Keywords
none
MeSH Terms
  • Animals
  • Cell Nucleus/physiology
  • Cell Nucleus/ultrastructure
  • Embryonic Development/physiology*
  • Image Interpretation, Computer-Assisted/methods
  • Imaging, Three-Dimensional/methods*
  • Microscopy, Fluorescence, Multiphoton/methods*
  • Reproducibility of Results
  • Sensitivity and Specificity
  • Zebrafish/anatomy & histology*
  • Zebrafish/embryology*
  • Zebrafish/physiology
PubMed
19964254 Full text @ Conf. Proc. IEEE Eng. Med. Biol. Soc.
Abstract
We present a simple and parameter-free nuclei tracking method for reconstructing cell dynamics in fluorescence 3D+t images of embryogenesis. The strategy is based on the use of the mathematical morphology operators directly in the 4D image. The morphological reconstruction of a marker -manually or automatically selected- in an initial spatio-temporal position generates a connected path over the time representing the cell migration. Thus, the processing provides a coherent spatiotemporal estimation of cell movement. The algorithm has been validated on in vivo images of early zebrafish and sea urchin embryogenesis acquired with two-photon laser scanning microscopy providing mean tracking rates above 98% per time step.
Genes / Markers
Figures
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping