PUBLICATION
Cell tracking in fluorescence images of embryogenesis processes with morphological reconstruction by 4D-tubular structuring elements
- Authors
- Pastor, D., Luengo-Oroz, M.A., Lombardot, B., Gonzalvez, I., Duloquin, L., Savy, T., Bourgine, P., Peyrieras, N., and Santos, A.
- ID
- ZDB-PUB-091215-46
- Date
- 2009
- Source
- Conference proceedings : ... Annual International Conference of the IEEE Engineering in Medicine and Biology Society. IEEE Engineering in Medicine and Biology Society. Annual Conference 1: 970-973 (Journal)
- Registered Authors
- Duloquin, Louise, Savy, Thierry
- Keywords
- none
- MeSH Terms
-
- Animals
- Cell Nucleus/physiology
- Cell Nucleus/ultrastructure
- Embryonic Development/physiology*
- Image Interpretation, Computer-Assisted/methods
- Imaging, Three-Dimensional/methods*
- Microscopy, Fluorescence, Multiphoton/methods*
- Reproducibility of Results
- Sensitivity and Specificity
- Zebrafish/anatomy & histology*
- Zebrafish/embryology*
- Zebrafish/physiology
- PubMed
- 19964254 Full text @ Conf. Proc. IEEE Eng. Med. Biol. Soc.
Citation
Pastor, D., Luengo-Oroz, M.A., Lombardot, B., Gonzalvez, I., Duloquin, L., Savy, T., Bourgine, P., Peyrieras, N., and Santos, A. (2009) Cell tracking in fluorescence images of embryogenesis processes with morphological reconstruction by 4D-tubular structuring elements. Conference proceedings : ... Annual International Conference of the IEEE Engineering in Medicine and Biology Society. IEEE Engineering in Medicine and Biology Society. Annual Conference. 1:970-973.
Abstract
We present a simple and parameter-free nuclei tracking method for reconstructing cell dynamics in fluorescence 3D+t images of embryogenesis. The strategy is based on the use of the mathematical morphology operators directly in the 4D image. The morphological reconstruction of a marker -manually or automatically selected- in an initial spatio-temporal position generates a connected path over the time representing the cell migration. Thus, the processing provides a coherent spatiotemporal estimation of cell movement. The algorithm has been validated on in vivo images of early zebrafish and sea urchin embryogenesis acquired with two-photon laser scanning microscopy providing mean tracking rates above 98% per time step.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping