PUBLICATION
Replacement of a single cysteine in the fourth transmembrane region of zebrafish pannexin1 alters hemichannel gating behavior
- Authors
- Prochnow, N., Hoffmann, S., Dermietzel, R., and Zoidl, G.
- ID
- ZDB-PUB-090828-17
- Date
- 2009
- Source
- Experimental brain research 199(3-4): 255-264 (Journal)
- Registered Authors
- Zoidl, Georg
- Keywords
- Electrical synapse, Pannexin, Connexin, Retina, Electrophysiology
- MeSH Terms
-
- Amino Acid Substitution/genetics*
- Animals
- Cell Line, Tumor
- Connexins/chemistry
- Connexins/genetics*
- Connexins/metabolism*
- Cysteine/genetics*
- Ion Channel Gating/genetics*
- Membrane Potentials/genetics
- Mice
- Mutagenesis, Site-Directed
- Protein Structure, Tertiary/genetics
- Zebrafish
- Zebrafish Proteins/chemistry
- Zebrafish Proteins/genetics*
- Zebrafish Proteins/metabolism*
- PubMed
- 19701745 Full text @ Exp. Brain Res.
Citation
Prochnow, N., Hoffmann, S., Dermietzel, R., and Zoidl, G. (2009) Replacement of a single cysteine in the fourth transmembrane region of zebrafish pannexin1 alters hemichannel gating behavior. Experimental brain research. 199(3-4):255-264.
Abstract
Pannexin1 (Panx1) is a novel candidate for an electrical synapse protein in the retina. At present Panx1 is considered to function as a hemichannel. Since information about the gating properties of Panx1 channels to date rely on blocker pharmacology, we have begun to establish a structural context of channel function starting with site directed mutagenesis of cysteine residues in transmembrane domains of Panx1. Dye uptake and whole cell voltage clamp recordings of transfected N2a cells demonstrate that zfPanx1 forms voltage activated hemichannels with a large unitary conductance in vitro. The function of this channel was significantly reduced following mutation of a single cysteine residue (C282W) in the fourth transmembrane region. This result suggests a role of this domain in gating of the Panx1 hemichannel.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping